亚热带资源与环境学报
亞熱帶資源與環境學報
아열대자원여배경학보
JOURNAL OF SUBTROPICAL RESOURCES AND ENVIRONMENT
2013年
1期
56-60
,共5页
詹旋灿%苏玉萍*%庄一廷%陈友震%鄢晶
詹鏇燦%囌玉萍*%莊一廷%陳友震%鄢晶
첨선찬%소옥평*%장일정%진우진%언정
沉积物%氮%释放%微生物%杜塘水库
沉積物%氮%釋放%微生物%杜塘水庫
침적물%담%석방%미생물%두당수고
sediment%nitrogen%nitrogen release%microbe%Dutang reservoir
本研究以杜塘水库大坝断面沉积物为对象,通过对上覆水—沉积物界面的好氧/厌氧连续培养模拟试验,比较灭菌与非灭菌条件下,上覆水中氨氮和硝酸盐氮的浓度变化以及沉积物中氨氮和总氮质量分数的变化,并分析培养前后沉积物中与氮循环相关微生物的变化,研究微生物对氮释放的影响.结果表明,灭菌组沉积物氨氮和硝酸盐主要通过上覆水—沉积物浓度梯度作用力进行扩散释放,较快达到平衡;而未灭菌组氨氮和硝酸盐的释放受溶解氧浓度影响,缺氧环境在微生物的作用下会促进反硝化作用以及氨氮的释放,沉积物中含有104 CFU/g的氨化细菌和反硝化细菌,暗示着沉积物中微生物作用下的氨化作用和反硝化作用较为激烈.
本研究以杜塘水庫大壩斷麵沉積物為對象,通過對上覆水—沉積物界麵的好氧/厭氧連續培養模擬試驗,比較滅菌與非滅菌條件下,上覆水中氨氮和硝痠鹽氮的濃度變化以及沉積物中氨氮和總氮質量分數的變化,併分析培養前後沉積物中與氮循環相關微生物的變化,研究微生物對氮釋放的影響.結果錶明,滅菌組沉積物氨氮和硝痠鹽主要通過上覆水—沉積物濃度梯度作用力進行擴散釋放,較快達到平衡;而未滅菌組氨氮和硝痠鹽的釋放受溶解氧濃度影響,缺氧環境在微生物的作用下會促進反硝化作用以及氨氮的釋放,沉積物中含有104 CFU/g的氨化細菌和反硝化細菌,暗示著沉積物中微生物作用下的氨化作用和反硝化作用較為激烈.
본연구이두당수고대패단면침적물위대상,통과대상복수—침적물계면적호양/염양련속배양모의시험,비교멸균여비멸균조건하,상복수중안담화초산염담적농도변화이급침적물중안담화총담질량분수적변화,병분석배양전후침적물중여담순배상관미생물적변화,연구미생물대담석방적영향.결과표명,멸균조침적물안담화초산염주요통과상복수—침적물농도제도작용력진행확산석방,교쾌체도평형;이미멸균조안담화초산염적석방수용해양농도영향,결양배경재미생물적작용하회촉진반초화작용이급안담적석방,침적물중함유104 CFU/g적안화세균화반초화세균,암시착침적물중미생물작용하적안화작용화반초화작용교위격렬.
@@@@Nitrogen release from sediments was analyzed near the Dutang Reservoir Dam Section, through parallel experiments under aerobic and anaerobic conditions .Based on a series of tests inclu-ding sterilization and non-sterilization conditions in simulated water-sediment reactors, concentrations of ammonia nitrogen and nitrate in overlying water and mass fraction of ammonia nitrogen and total nitrogen in sediments were determined.In addition, the change of related microbes of nitrogen cycle before and after cultivation was analyzed .Results showed that ammonia nitrogen and nitrate in the sed -iments released mainly through the force caused by concentration gradient , and the balance could be achieved faster under sterilized condition.However, the release of ammonia nitrogen and nitrate would be at the same time affected by dissolved oxygen (DO) concentration under non -sterilized condition, and the denitrification and ammonia nitrogen releasing be promoted by microbes under anoxic condi -tion.The number of ammonification and denitrifying bacteria in the sediments reaches 10 4 CFU per gram, implying that ammonification and denitrification processes are active under influences of microbes.