CAJ | 학술논문

目的探讨鼠双微体基因2(MDM2)启动子309位点单核苷酸多态性(SNP)与稽留流产的关系.方法对40例稽留流产妇女(研究组)的绒毛标本进行聚合酶链反应限制性片段长度多态性分析(PCR-RFLP),与40例健康早期妊娠妇女(对照组)的绒毛标本进行PCR-RFLP对照.结果根据显性基因模型计算,研究组T/T型占27.5%(11/40),T/G型+G/G型占72.5%(29/40),对照组分别为35.0%(14/40)、65.0%(26/40),两组基因型分布比较,差异无统计学意义(P>0.05).根据隐性基因模型计算,研究组G/G型占32.5%(13/40),T/T型+T/G型占67.5%(27/40),对照组分别为12.5%(5/40)、87.5%(35/40),两组基因型分布比较,差异有统计学意义(P<0.05).结论MDM2 SNP309 G/G基因型与稽留流产的发病风险增加有关,其确切的分子机制有待进一步研究.
목적탐토서쌍미체기인2(MDM2)계동자309위점단핵감산다태성(SNP)여계류유산적관계.방법대40례계류유산부녀(연구조)적융모표본진행취합매련반응한제성편단장도다태성분석(PCR-RFLP),여40례건강조기임신부녀(대조조)적융모표본진행PCR-RFLP대조.결과근거현성기인모형계산,연구조T/T형점27.5%(11/40),T/G형+G/G형점72.5%(29/40),대조조분별위35.0%(14/40)、65.0%(26/40),량조기인형분포비교,차이무통계학의의(P>0.05).근거은성기인모형계산,연구조G/G형점32.5%(13/40),T/T형+T/G형점67.5%(27/40),대조조분별위12.5%(5/40)、87.5%(35/40),량조기인형분포비교,차이유통계학의의(P<0.05).결론MDM2 SNP309 G/G기인형여계류유산적발병풍험증가유관,기학절적분자궤제유대진일보연구.
@@@@Objective To explore the association between single nucleotide polymorphism (SNP) of murine double minute 2(MDM2)-309 and missed abortion.Methods Genotyping of MDM 2 309 polymorphism was conducted by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) with villous samples collected from 40 women confirmed as missed abortion (study group) and from 40 healthy women with early pregnancy (control group). Results Calculation was performed following a dominant genotype model ,and we found that T/T genotype accounted for 27.50%(11/40) in the study group,and T/G plus G/G genotypes accounted for 72.50%(29/40),wherase in the control group,these figures were 35.0%(14/40) and 65.00%(26/40),respectively.No significant difference was found between two groups in the distribution of genotypes (P>0.05).When calculation was performed based on a recessive genotype model,we found that G/G genotype accounted for 32.50%(13/40) in the study group,and T/T plus T/G genotypes accounted for 67.50%(27/40),wherase in the control group,these figures were 12.50%(5/40) and 87.50%(35/40),respectively.There was a significant difference in the distribution of genotypes between two groups (P<0.05).Conclusion The MDM2 SNP309 G/G genotype is associated with an increased risk of missed abortion.Further study on its molecular mechanism is warranted .