CAJ | 학술논문

目的探讨顺铂联合低分子柑橘果胶(LCP)对人肺癌细胞的增殖及凋亡通路的影响.方法采用四甲基偶氮唑盐法检测各浓度顺铂单药(单药组)、顺铂联合LCP(联合组)对人肺癌细胞A549增殖的影响,用流式细胞仪检测半抑制浓度顺铂单药及联合用药处理A549细胞的凋亡比例,采用Western blot分析凋亡相关蛋白procaspase-3、8、9的变化.结果顺铂单药与联合LCP用药对A549细胞生长均有抑制作用,其效应呈时间和浓度依赖性;联合组对A549细胞的生长抑制作用更为显著(P<0.01).单药与联合用药均能使A549细胞凋亡比例增加,联合组较单药组细胞凋亡更加显著.单药组与联合组A549细胞的凋亡相关蛋白procaspase-3、8、9蛋白的表达均下调,联合组较单药组procaspase-3、9蛋白表达下调更为显著,但两组procaspase-8蛋白表达无明显差异.结论LCP能增加顺铂的细胞增殖抑制和诱导凋亡能力,该效应可能与活化线粒体凋亡途径有关.
목적탐토순박연합저분자감귤과효(LCP)대인폐암세포적증식급조망통로적영향.방법채용사갑기우담서염법검측각농도순박단약(단약조)、순박연합LCP(연합조)대인폐암세포A549증식적영향,용류식세포의검측반억제농도순박단약급연합용약처리A549세포적조망비례,채용Western blot분석조망상관단백procaspase-3、8、9적변화.결과순박단약여연합LCP용약대A549세포생장균유억제작용,기효응정시간화농도의뢰성;연합조대A549세포적생장억제작용경위현저(P<0.01).단약여연합용약균능사A549세포조망비례증가,연합조교단약조세포조망경가현저.단약조여연합조A549세포적조망상관단백procaspase-3、8、9단백적표체균하조,연합조교단약조procaspase-3、9단백표체하조경위현저,단량조procaspase-8단백표체무명현차이.결론LCP능증가순박적세포증식억제화유도조망능력,해효응가능여활화선립체조망도경유관.
@@@@Objective To study the effects of DDP combined with low-molecular-weight citrus pectin(LCP) on proliferation and apoptosis of human lung carcinoma cell .Methods The effects of DDP with(DDP group) or without LCP(combination group) in different concentrations on the proliferation of human lung carcinoma cell line A 549 were determined by MTT assay ,and the cell apoptosis proportion of A549 cell treated with DDP,DDP and LCP(IC50) was analyzed by facial action coding system(FACS).The changes of cell apoptosis-related proteins including procaspase-3,8,9 were measured with Western blot.Results DDP inhibited A549 cell proliferation in a time-and dose-dependent manner as well as DDP combined with LCP;The inhibitory effect of combination group on A549 cell proliferation was more significant(P<0.01).DDP group and combination group could increase the apoptosis proportion of A 549 cell,and the increase was more significant in the combination group .Expressions of cell apoptosis-related proteins including procaspase-3,8,9 all decreased in two groups,and the down-regulation of procaspase-3,9 protein expressions in the combination group was more significant than that in the DDP group,but procaspase-8 protein expression showed no significant difference between two groups.Conclusion LCP can enhance the ability of DDP to inhibit cell proliferation and induce cell apoptosis,which may be related to the activation of mitochondrial apoptosis pathway.