CAJ | 학술논문

背景:胰岛移植为有望根治糖尿病的一种方法,而移植排斥问题阻碍了移植的开展,诱导受体对供体移植物的免疫耐受是解决同种异体移植排斥反应的关键.目的:观察骨髓间充质干细胞输注对糖尿病小型猪同种异体胰岛移植物存活时间的影响.方法:无菌条件下抽取动物骨髓,离心弃上清.取单核细胞层,洗涤后加入 L-DMEM 培养基,调整细胞浓度为1×109 L-1.培养48 h 后换液去除未贴壁的细胞,依据贴壁培养法分离和扩增间充质干细胞.取第5代细胞作为移植供源,调整细胞浓度为1×1010 L-1.四氧嘧啶制备糖尿病小型猪模型,随机分为骨髓细胞组和干细胞+骨髓细胞组,经肝门静脉分别植入胰岛细胞、骨髓细胞,胰岛细胞、骨髓细胞和骨髓间充质干细胞.结果与结论:骨髓间充质干细胞联合骨髓细胞输注较单纯骨髓细胞输注能显著延长同种异体小型猪胰岛移植物存活时间(P <0.05),同时受体血清胰岛素能较长时间维持较高水平.提示骨髓间充质干细胞输注能显著延长猪胰岛移植物存活时间,并促进胰岛移植物正常功能的发挥.
배경:이도이식위유망근치당뇨병적일충방법,이이식배척문제조애료이식적개전,유도수체대공체이식물적면역내수시해결동충이체이식배척반응적관건.목적:관찰골수간충질간세포수주대당뇨병소형저동충이체이도이식물존활시간적영향.방법:무균조건하추취동물골수,리심기상청.취단핵세포층,세조후가입 L-DMEM 배양기,조정세포농도위1×109 L-1.배양48 h 후환액거제미첩벽적세포,의거첩벽배양법분리화확증간충질간세포.취제5대세포작위이식공원,조정세포농도위1×1010 L-1.사양밀정제비당뇨병소형저모형,수궤분위골수세포조화간세포+골수세포조,경간문정맥분별식입이도세포、골수세포,이도세포、골수세포화골수간충질간세포.결과여결론:골수간충질간세포연합골수세포수주교단순골수세포수주능현저연장동충이체소형저이도이식물존활시간(P <0.05),동시수체혈청이도소능교장시간유지교고수평.제시골수간충질간세포수주능현저연장저이도이식물존활시간,병촉진이도이식물정상공능적발휘.
@@@@BACKGROUND: Islet transplantation is a promising method used to radical y cure diabetes mel itus. The immune rejection caused by transplantation hinders the development of transplantation. The immune tolerance of recipients to donor grafts is a key to solving the problem of al otransplantation rejection reaction. OBJECTIVE: To investigate the influence of infusion of bone marrow mesenchymal stem cel s on the survival time of islet al ograft in the minipigs after islet al otransplantation. METHODS: Under sterile condition, minipig bone marrow was taken and the supernatant was discarded after centrifugation. After washed with L-DMEM culture medium, mononuclear cel s were adjusted to be 1×109/L. After 48-hour culture, nonadherent cel s were discarded and mesenchymal stem cel s were isolated and amplified. Passage 5 cel s were taken as donors and cel concentration was adjusted to be 1×1010/L. Minipig models of al oxan-induced diabetes mel itus were prepared and then randomly divided into two groups: bone marrow cel s and bone marrow cel s + mesenchymal stem cel s. In the bone marrow cel s group, islet cel s and bone marrow cel s were infused. In the bone marrow cel s + mesenchymal stem cel s group, islet cel s, bone marrow cel s and bone marrow mesenchymal stem cel s were infused. RESULTS AND CONCLUSION: Compared with infusion of simple bone marrow cel s, infusion of bone marrow mesenchymal stem cel s and bone marrow cel s significantly prolonged the survival time of islet al ografts (P <0.05) and maintained high serum insulin level for a longer time in diabetic minipigs. These findings suggest that bone marrow mesenchymal stem cel infusion can significantly prolong the survival time of islet al ografts and contribute to the normal function of islet al ografts in diabetic minipigs.