CAJ | 학술논문

背景:骨碎补能在促进骨生长且取得了良好的临床疗效,其主要成分柚皮甙能否诱导骨髓间充质干细胞向成骨方向分化?目的:用柚皮甙诱导兔骨髓间充质干细胞向成骨方向分化.并观察柚皮甙诱导兔骨髓间充质干细胞向成骨方向分化的能力.方法:用贴壁筛选法对兔骨髓间充质干细胞进行分离培养.对生长良好的第3代骨髓间充质干细胞分别用50μg/L 的柚皮甙和经典成骨诱导剂向成骨方向进行诱导,分别进行成骨鉴定.结果与结论:经典成骨诱导液、柚皮甙诱导液都能使骨髓间充质干细胞向成骨方向分化,基质分泌增多,形成钙结节.用酶联免疫检测仪检测柚皮甙诱导组和经典成骨诱导组细胞吸光度值未见明显的差异.同时检测柚皮甙诱导液和 L-DMEN 细胞培养液的吸光度值发现两者差异无显著性意义(P >0.05).证实柚皮甙可成功诱导骨髓间充质干细胞向成骨方向分化,无明显毒性作用.
배경:골쇄보능재촉진골생장차취득료량호적림상료효,기주요성분유피대능부유도골수간충질간세포향성골방향분화?목적:용유피대유도토골수간충질간세포향성골방향분화.병관찰유피대유도토골수간충질간세포향성골방향분화적능력.방법:용첩벽사선법대토골수간충질간세포진행분리배양.대생장량호적제3대골수간충질간세포분별용50μg/L 적유피대화경전성골유도제향성골방향진행유도,분별진행성골감정.결과여결론:경전성골유도액、유피대유도액도능사골수간충질간세포향성골방향분화,기질분비증다,형성개결절.용매련면역검측의검측유피대유도조화경전성골유도조세포흡광도치미견명현적차이.동시검측유피대유도액화 L-DMEN 세포배양액적흡광도치발현량자차이무현저성의의(P >0.05).증실유피대가성공유도골수간충질간세포향성골방향분화,무명현독성작용.
@@@@BACKGROUND: Drynaria can promote bone growth and has made good clinical curative effect. Can the main ingredients of Naringin induce bone marrow measenchymal stem cel s to differentiation into osteoblasts? OBJECTIVE: Through the use of Naringin to induce osteogenic differentiation of rabbit bone marrow mesenchymal stem cel s, to observe the osteogenic ability of Naringin-induced rabbit bone marrow mesenchymal stem cel s. METHODS: Adherence screening method was used to separate and culture the rabbit bone marrow mesenchymal stem cel s. The wel -grown third generation of bone marrow measenchymal stem cel s were induced with 50 ug/L Naringin and classic osteogenic inducer respectively to differentiate into the osteoblasts, and the osteogenesis identification was performed. RESULTS AND CONCLUTION: Both the classic osteogenic inducer and Naringin induced liquid could induce the bone marrow mesenchymal stem cel s to differentiation into osteoblasts with increasing matrix secretion and calcium nodules formation. The enzyme-linked immunosorbent assay showed that there was no significant difference in absorbance value of the cel s between the Naringin induction group and the classic osteogenic inducer group. There was no significant difference in absorbance value of the cel s induced with Naringin induced liquid and L-DMEN culture medium (P > 0.05). The results indicate that Naringin can induce the bone marrow mesenchymal stem cel s to differentiate into osteoblasts without obvious toxic effect.