中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2013年
14期
2649-2655
,共7页
干细胞%干细胞学术探讨%间充质干细胞%来源%分离%非分化扩增%无血清%微载体悬浮培养%表型%分化%脐带%省级基金
榦細胞%榦細胞學術探討%間充質榦細胞%來源%分離%非分化擴增%無血清%微載體懸浮培養%錶型%分化%臍帶%省級基金
간세포%간세포학술탐토%간충질간세포%래원%분리%비분화확증%무혈청%미재체현부배양%표형%분화%제대%성급기금
stem cel s%stem cel academic discussion%mesenchymal stem cel s%source%isolation%non-differentiation expansion%serum-free%microcarrier suspending culture%phenotype%differentiation%umbilical cord%provincial grants-supported paper
背景:间充质干细胞是多能成体干细胞,对间充质干细胞来源、分离、培养的研究无论从观念上、研究方法上还是研究结果均不尽相同.目的:综述间充质干细胞分离、培养研究的新进展.方法:由作者应用计算机检索2007至2011年 PubMed 数据库,在英文标题和摘要中以“mesenchymal stem cel s”和“source, isolation or culture”检索,选择内容与间充质干细胞应用相关的文章,同一领域文献则选择近期发表或发表在权威杂志文章,共纳入43篇文献.结果与结论:间充质干细胞的规范化培养需要建立一套标准化的培养体系来实现间充质干细胞的分离、大量增殖.因此,需要进一步深入开展间充质干细胞的基础研究,建立体外扩增和向不同细胞定向诱导分化体系,开展间充质干细胞及转基因后移植的体内效应研究等均具有重要的意义.
揹景:間充質榦細胞是多能成體榦細胞,對間充質榦細胞來源、分離、培養的研究無論從觀唸上、研究方法上還是研究結果均不儘相同.目的:綜述間充質榦細胞分離、培養研究的新進展.方法:由作者應用計算機檢索2007至2011年 PubMed 數據庫,在英文標題和摘要中以“mesenchymal stem cel s”和“source, isolation or culture”檢索,選擇內容與間充質榦細胞應用相關的文章,同一領域文獻則選擇近期髮錶或髮錶在權威雜誌文章,共納入43篇文獻.結果與結論:間充質榦細胞的規範化培養需要建立一套標準化的培養體繫來實現間充質榦細胞的分離、大量增殖.因此,需要進一步深入開展間充質榦細胞的基礎研究,建立體外擴增和嚮不同細胞定嚮誘導分化體繫,開展間充質榦細胞及轉基因後移植的體內效應研究等均具有重要的意義.
배경:간충질간세포시다능성체간세포,대간충질간세포래원、분리、배양적연구무론종관념상、연구방법상환시연구결과균불진상동.목적:종술간충질간세포분리、배양연구적신진전.방법:유작자응용계산궤검색2007지2011년 PubMed 수거고,재영문표제화적요중이“mesenchymal stem cel s”화“source, isolation or culture”검색,선택내용여간충질간세포응용상관적문장,동일영역문헌칙선택근기발표혹발표재권위잡지문장,공납입43편문헌.결과여결론:간충질간세포적규범화배양수요건립일투표준화적배양체계래실현간충질간세포적분리、대량증식.인차,수요진일보심입개전간충질간세포적기출연구,건입체외확증화향불동세포정향유도분화체계,개전간충질간세포급전기인후이식적체내효응연구등균구유중요적의의.
@@@@BACKGROUND: Mesenchymal stem cel s are multipotent adult stem cel s. The source, isolation and culture of mesenchymal stem cel s differ greatly in different studies in terms of concepts, research methods and outcomes. OBJECTIVE: To investigate the progress in isolation and culture of mesenchymal stem cel s. METHODS: The PubMed database (http://www.ncbi.nim.nih.gov/PubMed) was retrieved for articles regarding application of stem cel s from 2007 to 2011 by computer. The English key words are“mesenchymal stem cel s” and “source isolation or culture”. The papers published recently or in authoritative journals were selected. Forty-three papers were suitable for final analysis. RESULTS AND CONCLUSION: A standardized culture system should be established to achieve isolation and expansion in vitro of mesenchymal stem cel s. Therefore, basic research of mesenchymal stem cel s should be further developed, and the system of in vitro expansion and oriented differentiation of mesenchymal stem cel s should be established, and study on the effects of transplantation of gene-transfected mesenchymal stem cel s is of great significance.
