CAJ | 학술논문

本研究目的为考察在酵母粉或Cu2+胁迫下,古菌Ferroplasma thermophilum在分别与其他两种中度嗜热细菌(Leptospirillum ferriphilum和Acidithiobacillus caldus)混合培养体系中的生态功能.实验在250 mL摇瓶中进行,采用纯培养与混合培养对照的方法,结合荧光定量PCR(Quantitive PCR, Q-PCR)技术考察F. thermophilum分别与其他两种中度嗜热菌之间的协同作用.结果表明:在0.04%(w/v)酵母粉胁迫下,L. ferriphilum YSK纯培养物的生长受到完全抑制,而At. caldus s2纯培养物的生长不受抑制.F. thermophilum L1与L. ferriphilum YSK混合培养减轻了酵母粉对L. ferriphilum YSK的抑制作用且促进其生长;F. thermophilum L1/At. caldus s2混合培养体系中, F. thermophilum L1的加入能够促进平稳期At. caldus s2的生长.在5 g/L Cu2+胁迫下,F. thermophilum L1与L. ferriphilum YSK混合培养时,F. thermophilum L1生长被促进,而L. ferriphilum YSK的生长受到抑制; F. thermophilum L1与At. caldus s2混合培养能够有效地促进彼此生长,且协同促进铁/硫氧化代谢.
본연구목적위고찰재효모분혹Cu2+협박하,고균Ferroplasma thermophilum재분별여기타량충중도기열세균(Leptospirillum ferriphilum화Acidithiobacillus caldus)혼합배양체계중적생태공능.실험재250 mL요병중진행,채용순배양여혼합배양대조적방법,결합형광정량PCR(Quantitive PCR, Q-PCR)기술고찰F. thermophilum분별여기타량충중도기열균지간적협동작용.결과표명:재0.04%(w/v)효모분협박하,L. ferriphilum YSK순배양물적생장수도완전억제,이At. caldus s2순배양물적생장불수억제.F. thermophilum L1여L. ferriphilum YSK혼합배양감경료효모분대L. ferriphilum YSK적억제작용차촉진기생장;F. thermophilum L1/At. caldus s2혼합배양체계중, F. thermophilum L1적가입능구촉진평은기At. caldus s2적생장.재5 g/L Cu2+협박하,F. thermophilum L1여L. ferriphilum YSK혼합배양시,F. thermophilum L1생장피촉진,이L. ferriphilum YSK적생장수도억제; F. thermophilum L1여At. caldus s2혼합배양능구유효지촉진피차생장,차협동촉진철/류양화대사.
@@@@The purpose of this study was to explore the ecological functions of Ferroplasma thermophilum when it was mixed with Leptospirillum ferriphilum or Acidithiobacillus caldus under the stress of yeast extract or Cu2+. Population dynamics of pure culture and mixed culture were compared by using Quantitative PCR, and synergies between Ferroplasma thermophilum and the other two moderately thermophilic bacteria were evaluated. The results show that the growth of pure L. ferriphilum YSK is inhibited under the stress of 0.04% (w/v) yeast extract. Yet, L. ferriphilum YSK grows well in the presence of F. thermophilum L1, which indicates that the toxicity of yeast extract to L. ferriphilum YSK is reduced in the mixed culture. The results also show that there is no influence of yeast extract on the growth of At. caldus s2 in the pure culture, and compared with the pure culture, the cell density of At. caldus s2 at the stationary phase increases in the mixed culture. Under the stress of 5 g/L Cu2+, when F. thermophilum L1 and L. ferriphilum YSK are mix-cultured, the growth of F. thermophilum L1 can be promoted, while the growth of L. ferriphilum YSK is inhibited. The mixed culture of F. thermophilum and At. caldus has a positive effect on the growth of each other, and can mutually promote ferrous or sulfur oxidation under the stress of 5 g/L Cu2+.