CAJ | 학술논문

目的:探讨大肠癌中是否存在血管生成拟态(VM),阐述VM存在的临床意义;分析VM与αvβ3 integrin表达的关系;研究大肠癌中αvβ3 integrin表达的临床意义及其与FAK、MMP-2、VEGF表达的相关性.方法:对227例大肠癌组织切片进行CD31免疫组织化学和PAS染色及αvβ3 integrin、FAK、VEGF、MMP-2免疫组织化学染色.结果:VM在大肠癌中阳性率为18.06%(41/227),αvβ3 integrin在大肠癌中阳性率为56.83%(129/227);VM阳性组、αvβ3 integrin阳性组生存时间均较阴性组短,差异均有统计学意义(P<0.05);VM阳性组的αvβ3 integrin阳性率较阴性组高,差异有统计学意义(P<0.05);αvβ3 integrin与FAK、VEGF、MMP-2表达呈正相关.结论:大肠癌中VM的存在、αvβ3 integrin的表达与肿瘤的不良预后有关;αvβ3 integrin可能通过与FAK、VEGF、MMP-2的相互作用,参与了大肠癌VM的形成.
목적:탐토대장암중시부존재혈관생성의태(VM),천술VM존재적림상의의;분석VM여αvβ3 integrin표체적관계;연구대장암중αvβ3 integrin표체적림상의의급기여FAK、MMP-2、VEGF표체적상관성.방법:대227례대장암조직절편진행CD31면역조직화학화PAS염색급αvβ3 integrin、FAK、VEGF、MMP-2면역조직화학염색.결과:VM재대장암중양성솔위18.06%(41/227),αvβ3 integrin재대장암중양성솔위56.83%(129/227);VM양성조、αvβ3 integrin양성조생존시간균교음성조단,차이균유통계학의의(P<0.05);VM양성조적αvβ3 integrin양성솔교음성조고,차이유통계학의의(P<0.05);αvβ3 integrin여FAK、VEGF、MMP-2표체정정상관.결론:대장암중VM적존재、αvβ3 integrin적표체여종류적불량예후유관;αvβ3 integrin가능통과여FAK、VEGF、MMP-2적상호작용,삼여료대장암VM적형성.
This work aimed to investigate the expression and clinical significance of vasculogenic mimicry (VM), to analyze the relationship between VM andαvβ3 integrin expressions, and to study the clinical significance ofαvβ3 integrin expression and its correlation with the Focal Adhesion Kinase (FAK), Vascular Endothelial Growth Factor (VEGF), and Matrix Metalloproteinase 2 (MMP-2) expression. Methods:VM expression was observed by CD31 immunohistochemical and PAS staining. Expression ofαvβ3 integrin, FAK, VEGF, and MMP-2 was observed by immunohistochemical staining. Results:Results showed that VM andαvβ3 integ-rin had positive rates of 18.06%(41/227) and 56.83%(129/227), respectively. Patients with VM expression had a shorter survival time than those without VM expression (P<0.05).αvβ3 integrin-positive patients had a shorter survival time thanαvβ3 integrin-negative pa-tients (P<0.05). Patients with VM expression showed a higher positive rate of αvβ3 integrin than those without VM expression (P<0.05), andαvβ3 integrin had a positive correlation with FAK, MMP-2, and VEGF expression. Conclusion:The incidence of VM andαvβ3 integrin expressions in human colorectal cancer cases indicates a poor prognosis.αvβ3 integrin possibly participates in the formation of VM through interactions with FAK, VEGF, and MMP-2.