中国肿瘤临床
中國腫瘤臨床
중국종류림상
CHINESE JOURNAL OF CLINICAL ONCOLOGY
2013年
10期
575-578
,共4页
姜孝芳%李卉%刘玲%王洪江%李秀梅%陈艳%庞作良%谌宏鸣%尹娜%李艳%沙亚哈提·别尔克哈之%李惠武
薑孝芳%李卉%劉玲%王洪江%李秀梅%陳豔%龐作良%諶宏鳴%尹娜%李豔%沙亞哈提·彆爾剋哈之%李惠武
강효방%리훼%류령%왕홍강%리수매%진염%방작량%심굉명%윤나%리염%사아합제·별이극합지%리혜무
食管癌%Lrig1%NF-κB%MMP-2%哈萨克族
食管癌%Lrig1%NF-κB%MMP-2%哈薩剋族
식관암%Lrig1%NF-κB%MMP-2%합살극족
esophageal squamous cell carcinoma%Lrig1%NF-κB%MMP-2%Hazakhan
目的:探讨Lrig1、NF-κB及MMP-2在哈萨克族食管癌患者中的表达与PI3K-AKT、MEK-ERK信号通路及与临床病理指标之间的关系.方法:采用RT-PCR方法检测48例哈萨克族食管癌患者组织中Lrig1、NF-κB、MMP-2表达,探讨NF-κB、MMP-2的表达与Lrig1的关系.纳入PI3K及MEK信号通路的关键基因PI3K、AKT1、MEK1、MEK2、MEK5、ERK1、ERK2,研究Lrig1调控NF-κB、MMP-2基因表达的通路.结果:转录水平mRNA检测显示在肿瘤组织及远端正常组织中NF-κB(P<0.001,P=0.014)、MMP-2(P=0.003,P=0.045)的表达与Lrig1 mRNA表达相关;蛋白水平Western blot检测显示Lrig1与NF-κB、MMP-2可能呈负相关.在肿瘤组织中MEK5(P<0.001)及ERK2(P=0.009)的表达与Lrig1相关;PI3K在正常组织中(P<0.001)的表达与Lrig1相关.Lrig1、MMP-2及NF-κB协同表达率为52.1%(25/48),其协同表达与淋巴结转移相关(P=0.020).结论:NF-κB、MMP-2与Lrig1协同表达促进食管癌患者的淋巴结转移,Lrig1可能是通过MEK-ERK信号通路调控相关基因表达.
目的:探討Lrig1、NF-κB及MMP-2在哈薩剋族食管癌患者中的錶達與PI3K-AKT、MEK-ERK信號通路及與臨床病理指標之間的關繫.方法:採用RT-PCR方法檢測48例哈薩剋族食管癌患者組織中Lrig1、NF-κB、MMP-2錶達,探討NF-κB、MMP-2的錶達與Lrig1的關繫.納入PI3K及MEK信號通路的關鍵基因PI3K、AKT1、MEK1、MEK2、MEK5、ERK1、ERK2,研究Lrig1調控NF-κB、MMP-2基因錶達的通路.結果:轉錄水平mRNA檢測顯示在腫瘤組織及遠耑正常組織中NF-κB(P<0.001,P=0.014)、MMP-2(P=0.003,P=0.045)的錶達與Lrig1 mRNA錶達相關;蛋白水平Western blot檢測顯示Lrig1與NF-κB、MMP-2可能呈負相關.在腫瘤組織中MEK5(P<0.001)及ERK2(P=0.009)的錶達與Lrig1相關;PI3K在正常組織中(P<0.001)的錶達與Lrig1相關.Lrig1、MMP-2及NF-κB協同錶達率為52.1%(25/48),其協同錶達與淋巴結轉移相關(P=0.020).結論:NF-κB、MMP-2與Lrig1協同錶達促進食管癌患者的淋巴結轉移,Lrig1可能是通過MEK-ERK信號通路調控相關基因錶達.
목적:탐토Lrig1、NF-κB급MMP-2재합살극족식관암환자중적표체여PI3K-AKT、MEK-ERK신호통로급여림상병리지표지간적관계.방법:채용RT-PCR방법검측48례합살극족식관암환자조직중Lrig1、NF-κB、MMP-2표체,탐토NF-κB、MMP-2적표체여Lrig1적관계.납입PI3K급MEK신호통로적관건기인PI3K、AKT1、MEK1、MEK2、MEK5、ERK1、ERK2,연구Lrig1조공NF-κB、MMP-2기인표체적통로.결과:전록수평mRNA검측현시재종류조직급원단정상조직중NF-κB(P<0.001,P=0.014)、MMP-2(P=0.003,P=0.045)적표체여Lrig1 mRNA표체상관;단백수평Western blot검측현시Lrig1여NF-κB、MMP-2가능정부상관.재종류조직중MEK5(P<0.001)급ERK2(P=0.009)적표체여Lrig1상관;PI3K재정상조직중(P<0.001)적표체여Lrig1상관.Lrig1、MMP-2급NF-κB협동표체솔위52.1%(25/48),기협동표체여림파결전이상관(P=0.020).결론:NF-κB、MMP-2여Lrig1협동표체촉진식관암환자적림파결전이,Lrig1가능시통과MEK-ERK신호통로조공상관기인표체.
This work aimed to investigate the expression levels of Lrig1, NF-κB, and MMP-2 in Hazak's esophageal squamous cell carcinoma (ESCC), as well as to explore the relationship of Lrig1 expression through the PI3K-AKT and MEK-ERK signaling pathways with clinicopathological indices. Methods:First, RT-PCR analysis was performed to study the expression levels of Lrig1, NF-κB, and MMP-2 in 48 ESCC and noncancerous specimens. Second, some key genes of the PI3K and MEK signaling pathways such as PI3K, AKT1, MEK1, MEK2, MEK5, ERK1, and ERK2 were detected in Lrig1-positive and-negative tissues to identify the possible signaling pathway of Lrig1-regulated NF-κB and MMP-2 expression. Results:The expression of NF-κB (P<0.001, P=0.014) and MMP-2 (P=0.003, P=0.045) was significantly correlated with Lrig1 in cancer and distal normal tissues. Lrig1 may be negatively correlated with NF-κB and MMP-2 at the protein level. MEK5 (P<0.001) and ERK2 (P=0.009) expression was associated with Lrig1 in cancer tissues. PI3K expression was correlated with Lrig1 in the distal normal tissues (P<0.001). The coordinate expression ratio of Lrig1 to NF-κB and MMP2 reached 52.1%(25/48). This co-expression may be related to lymph node metastasis (P=0.020) but not to other clinicopathological features. Conclusions:These findings suggest that MMP-2 and NF-κB expression is related to Lrig1 in Hazak's ESCC, and that MEK and ERK2 mRNA expression are related to Lrig1. The co-expression of NF-κB, MMP-2, and Lrig1 may promote lymph node metastasis in ESCC among Hazak people. Lrig1 may regulate the expression of target genes not through the PI3K-AKT pathway but through the MEK-ERK signaling pathway. Further related studies are needed in the future.
