EphB4及其配体EphrinB2在食管鳞癌中表达及其与血管生成的关系*
EphB4급기배체EphrinB2재식관린암중표체급기여혈관생성적관계*
Expression of EphB4 receptor and its ligand EphrinB2 in esophageal cancer tissues and their relationship with tumor angiogenesis
  • 万方数据
    中国肿瘤临床 중국종류림상
    CHINESE JOURNAL OF CLINICAL ONCOLOGY
    2013年 10期 579-582 ,共4页

    郑庆丰%柳硕岩%王海燕%王枫%王镇%陈啸风%王健键%应敏刚%郑雄伟%林贤东%周智锋%龚福生%谢云青

    정경봉%류석암%왕해연%왕풍%왕진%진소풍%왕건건%응민강%정웅위%림현동%주지봉%공복생%사운청

    EphB4%EphrinB2%食管%鳞状细胞癌%血管生成 EphB4%EphrinB2%食管%鱗狀細胞癌%血管生成
    EphB4%EphrinB2%식관%린상세포암%혈관생성
    EphB4%EphrinB2%Esophagus%squamous cell carcinoma%angiogenesis
    目的:探讨EphB4受体及其配体EphrinB2在食管鳞癌组织中的表达及其与血管生成的关系.方法:应用免疫组织化学Envision plus法检测60例食管鳞癌和10例癌旁组织中EphB4受体及其配体EphrinB2的表达和VEGF的表达,采用逆转录多聚酶链反应(RT-PCR)法检测60例食管鳞癌和10例癌旁食管组织中EphB4、EphrinB2和VEGF mRNA的相对含量,并分析EphB4、Eph?rinB2和VEGF的表达的相关性.结果:食管鳞癌组织中EphB4、EphrinB2、VEGF mRNA和蛋白的表达均高于癌旁正常食管黏膜,差异有统计学意义(P<0.05).EphB4 mRNA和EphrinB2 mRNA表达正相关(r=0.541,P<0.05),EphB4 mRNA、EphrinB2 mRNA均和VEGF mRNA表达正相关(r=0.642,r=0.582;P均<0.05).结论:EphB4和EphrinB2在食管鳞癌中高表达,与血管生成密切相关.
    목적:탐토EphB4수체급기배체EphrinB2재식관린암조직중적표체급기여혈관생성적관계.방법:응용면역조직화학Envision plus법검측60례식관린암화10례암방조직중EphB4수체급기배체EphrinB2적표체화VEGF적표체,채용역전록다취매련반응(RT-PCR)법검측60례식관린암화10례암방식관조직중EphB4、EphrinB2화VEGF mRNA적상대함량,병분석EphB4、Eph?rinB2화VEGF적표체적상관성.결과:식관린암조직중EphB4、EphrinB2、VEGF mRNA화단백적표체균고우암방정상식관점막,차이유통계학의의(P<0.05).EphB4 mRNA화EphrinB2 mRNA표체정상관(r=0.541,P<0.05),EphB4 mRNA、EphrinB2 mRNA균화VEGF mRNA표체정상관(r=0.642,r=0.582;P균<0.05).결론:EphB4화EphrinB2재식관린암중고표체,여혈관생성밀절상관.
    This study aimed to investigate the expression of EphB4 receptor and its ligand EphrinB2 in esophageal squamous cell carcinoma and their relationship with angiogenesis. Methods:The expression of EphB4 receptor and its ligand EphrinB2 and VEGF was detected by immunohistochemistry in tumor specimens from 60 esophageal squamous cell carcinoma cases and paratumor normal specimens from 10 cases. The expression of EphB4, EphrinB2, and VEGF mRNA in 60 patients with esophageal cancer and 10 patients with paratumor normal esophageal mucosa was detected by reverse-transcription polymerase chain reaction. The correlation among the expression levels of EphB4, EphrinB2, and VEGF mRNA was analyzed. Results:The expression levels of EphB4, EphrinB2, and VEGF mRNA, as well as of the expression levels protein, were significantly higher than that in paratumor normal esophageal mucosa (P<0.05). The relative amount of EphB4 mRNA was positively correlated with EphrinB2 mRNA in patients with esophageal cancer (r=0.541, P<0.05). The relative amounts of EphB4 mRNA and EphrinB2 mRNA were positively correlated with VEGF mRNA in patients with esophageal cancer (r=0.642 and 0.582, respectively;P<0.05). Conclusion:EphB4 and EphrinB2 are highly expressed in esophageal cancer tissue, which may be closely associated with the angiogenesis of esophageal cancer.
    원문보기 원문다운로드 사이트로이동
저자의 최근 논문