CAJ | 학술논문

目的:观察兔脂肪源性干细胞分化及贴覆能力.方法:取家兔颈部皮下脂肪以酶消化法获取细胞,倒置显微镜下观察细胞生长情况,加入诱导培养液对所获得细胞进行成骨成脂诱导,并分别进行形态学、钙盐沉积和油红O染色相关染色鉴定.电镜下观察细胞贴覆支架的情况.结果:在诱导条件下,碱性磷酸酶及钙盐沉积均呈阳性表达,油红O染色为阳性.结论:脂肪干细胞有多向分化潜能,能很好地贴覆在支架上生长.
목적:관찰토지방원성간세포분화급첩복능력.방법:취가토경부피하지방이매소화법획취세포,도치현미경하관찰세포생장정황,가입유도배양액대소획득세포진행성골성지유도,병분별진행형태학、개염침적화유홍O염색상관염색감정.전경하관찰세포첩복지가적정황.결과:재유도조건하,감성린산매급개염침적균정양성표체,유홍O염색위양성.결론:지방간세포유다향분화잠능,능흔호지첩복재지가상생장.
Objective:To observe the differentiation and adhesion ability of adipose-derived stem cells (ADSCs). Methods:The ADSCs were isolated from rabbit cervical subcutaneous fat and puri-fied by enzyme digestion. The growth situation of ADSCs was observed under microscope. Adding induced nutrient solution to the cells to induce osteogenesis and adipogenesis. The passage cells were stained by Alkaline phosphatase、Calcium salt deposition and oil red O staining associated staining identification. Cells and scaffold composite situation were observed under the electron mi-croscopic. Results:In the induction condition,Alkaline phosphatase and calcium salt deposits were positive expression, Oil red O stain positive expression. Conclusions:ADSCs have multipotential differentiation potential,it can cover and grow on the bracket well.