CAJ | 학술논문

选用不同外植体对邓恩桉组培快繁的初代培养、继代培养、生根培养等各阶段条件进行研究.结果为:以截杆萌发的邓恩桉幼嫩芽条为外植体,采用 F +0.5 mg ? L -16-BA +0.3 mg ? L -1 IBA 培养基,有利于外植体的不定芽诱导以及初期的继代扩大培养,增殖系数达3.68;以改良 F +0.25 mg ? L -16-BA +0.25-0.5 mg ? L -1 IBA +20 mg ? L -1 VC +10 mg ? L -1 VB2为培养基,培养初期进行黑暗处理10 d ,增殖系数达3.26,小苗高度达1~2.5 cm ,适宜用于进一步的生根培养;以 DR 为邓恩桉生根基本培养基并添加蔗糖40 g ? L -1 +1 .0mg ? L -1ABT 1号生根粉,生根诱导率为84.67%.
선용불동외식체대산은안조배쾌번적초대배양、계대배양、생근배양등각계단조건진행연구.결과위:이절간맹발적산은안유눈아조위외식체,채용 F +0.5 mg ? L -16-BA +0.3 mg ? L -1 IBA 배양기,유리우외식체적불정아유도이급초기적계대확대배양,증식계수체3.68;이개량 F +0.25 mg ? L -16-BA +0.25-0.5 mg ? L -1 IBA +20 mg ? L -1 VC +10 mg ? L -1 VB2위배양기,배양초기진행흑암처리10 d ,증식계수체3.26,소묘고도체1~2.5 cm ,괄의용우진일보적생근배양;이 DR 위산은안생근기본배양기병첨가자당40 g ? L -1 +1 .0mg ? L -1ABT 1호생근분,생근유도솔위84.67%.
Different explants from Eucalyptus dunnii were selected to study the optimal conditions for growth at the stages of initial culture , subculture and rooting . The results indicated that adventitious buds from shoot segments of Eucalyptus dunnii could be induced ,as well as propagated at the initial subculture stage on a medi-um of F + 0 .5 mg ? L -1 6 - BA + 0 .3 mg ? L -1 IBA , with the multiplication coefficient being 3 .68 . Im-proved medium of F + 0 .25 mg ? L -1 6 - BA + 0 .25 ~ 0 .5 mg ? L -1 IBA + 20 mg ? L -1 VC + 10 mg ? L -1 VB 2 along with dark treatment for 10 days proved appropriate for sprout explants ,with the multiplication coeffi-cient and height of seedling being 3 .26 and 1 ~ 2 .5 cm , respectively . Medium of DR supplemented with 40 g ? L -1 sugar and 1 .0 mg ? L -1 1 # ABT rooting powder was used for Eucalyptus dunnii rooting culture ,with rooting rate of up to 84 .7% .