北方药学
北方藥學
북방약학
JOURNAL OF NORTH PHARMACY
2013年
1期
4-5
,共2页
伤科跌打丸%高效液相色谱法%丹皮酚%含量测定
傷科跌打汍%高效液相色譜法%丹皮酚%含量測定
상과질타환%고효액상색보법%단피분%함량측정
Shangkedieda pill%High performance liquid chromatography%Paeonol%Determination of content
目的:建立 HPLC 法测定伤科跌打丸小蜜丸中丹皮酚的含量.方法:以十八烷基硅烷键合硅胶为填充剂,流动相为甲醇-水(70∶30),流速为1.0ml/min,检测波长为274nm,柱温为室温.结果:丹皮酚在上述色谱条件下与其他组分有良好的分离效果,丹皮酚线性范围为0.0408~0.2448μg,r=0.9998,平均回收率为99.16%,RSD=0.47%.结论:定量方法简便、可靠、准确、专属性强,可用于该药品成分的含量测定.
目的:建立 HPLC 法測定傷科跌打汍小蜜汍中丹皮酚的含量.方法:以十八烷基硅烷鍵閤硅膠為填充劑,流動相為甲醇-水(70∶30),流速為1.0ml/min,檢測波長為274nm,柱溫為室溫.結果:丹皮酚在上述色譜條件下與其他組分有良好的分離效果,丹皮酚線性範圍為0.0408~0.2448μg,r=0.9998,平均迴收率為99.16%,RSD=0.47%.結論:定量方法簡便、可靠、準確、專屬性彊,可用于該藥品成分的含量測定.
목적:건립 HPLC 법측정상과질타환소밀환중단피분적함량.방법:이십팔완기규완건합규효위전충제,류동상위갑순-수(70∶30),류속위1.0ml/min,검측파장위274nm,주온위실온.결과:단피분재상술색보조건하여기타조분유량호적분리효과,단피분선성범위위0.0408~0.2448μg,r=0.9998,평균회수솔위99.16%,RSD=0.47%.결론:정량방법간편、가고、준학、전속성강,가용우해약품성분적함량측정.
Objective:To establish an HPLC method for the determination of paeonol in the Shangkedieda pill:Octadecylsilane bonded silica as a filler,the mobile phase was methanol - water (70:30), flow rate of 1.0ml/min, detection wavelength was 274nm and the column temperature was room temperature. Results: the paeonol In the abovechromatographic conditions and other components have a good separation and the paeonol linear range 0.0408~0.2448μg The r=0.9998,average recovery was 99.16%,RSD=0.47%. Conclusions:The method is simple, reliable, accurate,specific and can be used for the Shangkedieda pill a small Miwan of quality control.
