北方药学
北方藥學
북방약학
JOURNAL OF NORTH PHARMACY
2013年
2期
1
,共1页
八味獐牙菜丸%獐牙菜苦苷%高效液相色谱法
八味獐牙菜汍%獐牙菜苦苷%高效液相色譜法
팔미장아채환%장아채고감%고효액상색보법
Bawei Zhangyacai pill%Swertimarin%HPLC
目的:建立八味獐牙菜丸中獐牙菜苦苷的含量测定方法.方法:色谱柱为岛津 VP-ODS C18(250mm×4.6mm,5μm),流动相为甲醇-水(20∶80),检测波长为237nm.结果:獐牙菜苦苷进样量在0.12~0.60mg·ml-1范围内峰面积与浓度呈良好的线性关系,相关系数为0.9999,平均回收率为97.70%,RSD 值为1.85%(n=9).结论:方法快速,准确,重复性好,可作为八味獐牙菜丸的质量指标.
目的:建立八味獐牙菜汍中獐牙菜苦苷的含量測定方法.方法:色譜柱為島津 VP-ODS C18(250mm×4.6mm,5μm),流動相為甲醇-水(20∶80),檢測波長為237nm.結果:獐牙菜苦苷進樣量在0.12~0.60mg·ml-1範圍內峰麵積與濃度呈良好的線性關繫,相關繫數為0.9999,平均迴收率為97.70%,RSD 值為1.85%(n=9).結論:方法快速,準確,重複性好,可作為八味獐牙菜汍的質量指標.
목적:건립팔미장아채환중장아채고감적함량측정방법.방법:색보주위도진 VP-ODS C18(250mm×4.6mm,5μm),류동상위갑순-수(20∶80),검측파장위237nm.결과:장아채고감진양량재0.12~0.60mg·ml-1범위내봉면적여농도정량호적선성관계,상관계수위0.9999,평균회수솔위97.70%,RSD 치위1.85%(n=9).결론:방법쾌속,준학,중복성호,가작위팔미장아채환적질량지표.
Objective:To establish a HPLC method for the simultaneous determination of Swertimarin Bawei Zhangyacai pill. Methods:Column for Shimadzu VP-ODS C18(250mm×4.6mm,5μm),the mobile phase was methanol - water(20∶80), the detection wavelength was 237 nm. Results: Swertimarin injection volume in from 0.12 ~0.60mg·ml -1 range peak area and concentration showed a good linear relationship, the correlation coefficient of 0.9999,the average recovery rate was 97.70%, RSD is 1.85%(n=9). Conclusions:The method is fast,accurate, reproducible, and can be used as the indicator of the quality of the Bawei Zhangyacai pill.
