茶叶科学
茶葉科學
다협과학
2013年
1期
40-44
,共5页
夏道宗%张元君%倪达美%居梦婷
夏道宗%張元君%倪達美%居夢婷
하도종%장원군%예체미%거몽정
安吉白茶多糖%抗肿瘤%免疫调节%腹腔巨噬细胞%吞噬能力
安吉白茶多糖%抗腫瘤%免疫調節%腹腔巨噬細胞%吞噬能力
안길백다다당%항종류%면역조절%복강거서세포%탄서능력
anjibaicha polysaccharide%antitumor%immune regulation%peritoneal macrophage%phagocytic function
选用 MTT 法研究了安吉白茶多糖的体外抗肿瘤活性;采用建立小鼠移植瘤模型,通过测定安吉白茶多糖对荷瘤小鼠抑瘤率和腹腔巨噬细胞吞噬能力,研究其体内抗肿瘤活性和免疫调节作用.结果表明,安吉白茶多糖体外能显著抑制 S180细胞的生长,且具有剂量依赖关系.体内试验表明,与模型对照组相比,安吉白茶多糖对小鼠 S180实体瘤和 H22腹水型肝癌移植瘤的生长均有显著抑制作用(P<0.05),对荷瘤小鼠脾指数和胸腺指数也有较强增高作用.体内免疫调节试验结果显示,安吉白茶多糖可使荷 S180实体瘤小鼠腹腔巨噬细胞吞噬能力显著升高(P<0.05).
選用 MTT 法研究瞭安吉白茶多糖的體外抗腫瘤活性;採用建立小鼠移植瘤模型,通過測定安吉白茶多糖對荷瘤小鼠抑瘤率和腹腔巨噬細胞吞噬能力,研究其體內抗腫瘤活性和免疫調節作用.結果錶明,安吉白茶多糖體外能顯著抑製 S180細胞的生長,且具有劑量依賴關繫.體內試驗錶明,與模型對照組相比,安吉白茶多糖對小鼠 S180實體瘤和 H22腹水型肝癌移植瘤的生長均有顯著抑製作用(P<0.05),對荷瘤小鼠脾指數和胸腺指數也有較彊增高作用.體內免疫調節試驗結果顯示,安吉白茶多糖可使荷 S180實體瘤小鼠腹腔巨噬細胞吞噬能力顯著升高(P<0.05).
선용 MTT 법연구료안길백다다당적체외항종류활성;채용건립소서이식류모형,통과측정안길백다다당대하류소서억류솔화복강거서세포탄서능력,연구기체내항종류활성화면역조절작용.결과표명,안길백다다당체외능현저억제 S180세포적생장,차구유제량의뢰관계.체내시험표명,여모형대조조상비,안길백다다당대소서 S180실체류화 H22복수형간암이식류적생장균유현저억제작용(P<0.05),대하류소서비지수화흉선지수야유교강증고작용.체내면역조절시험결과현시,안길백다다당가사하 S180실체류소서복강거서세포탄서능력현저승고(P<0.05).
The antitumor activity of anjibaicha polysaccharide in vitro by MTT method was investigated by the authors. For the purpose of investigating the antitumor and immune regulation functions of anjibaicha polysaccharide in vivo, to establish the transplant tumor model of mice, and determine the inhibition rate on tumor cells growth and peritoneal macrophage phagocytic function of tumor-burdened mice by anjibaicha polysaccharide. The results showed that the sarcoma-180 growth could be significant inhibited by anjibaicha polysaccharide in vitro and showed dose-dependent relation ship. The test in vivo showed that anjibaicha polysaccharide has significant inhibitory effects on sarcoma-180 and ascitic hepatoma H22 growth in tumor-burdened mice (P<0.05). In addition, anjibaicha polysaccharide could increase thymus and spleen indexs in mice with sarcoma-180 and ascitic hepatoma H22 cells. Moreover, anjibaicha polysaccharide could significantly increase the peritoneal macrophage phagocytic function in sarcoma-180-burdened mice (P<0.05).
