东北农业大学学报
東北農業大學學報
동북농업대학학보
JOURNAL OF NORTHEAST AGRICULTURAL UNIVERSITY
2013年
1期
18-23
,共6页
金正勋%王露露%刘海英%徐振华%曲莹%沈鹏%张忠臣
金正勛%王露露%劉海英%徐振華%麯瑩%瀋鵬%張忠臣
금정훈%왕로로%류해영%서진화%곡형%침붕%장충신
粳稻%杂种后代%淀粉分支酶%基因表达
粳稻%雜種後代%澱粉分支酶%基因錶達
갱도%잡충후대%정분분지매%기인표체
japonica%hybrid progeny%branch enzyme%gene expression
选用籽粒直链淀粉含量有显著差异的亲本和杂种后代,分析籽粒灌浆过程中直链淀粉积累和淀粉分支酶活性以及淀粉分支酶基因mRNA表达量等变化特点.结果表明,直链淀粉含量高的亲本及后代在灌浆过程中合成和积累的直链淀粉量显著高于直链淀粉含量低的亲本及后代;直链淀粉含量高的亲本及后代的淀粉分支酶活性均比直链淀粉含量低的亲本及后代高,灌浆不同时期的籽粒直链淀粉含量与淀粉分支酶活性间均呈正相关;亲本及杂种后代籽粒OsSBE1和OsSBE3的mRNA表达量随灌浆进程逐渐增加,达到峰值后又逐渐下降,呈单峰曲线变化,其表达量峰值都出现在抽穗后17 d,在灌浆过程中胚乳OsSBE4基因的mRNA表达量相对比较小;杂种后代籽粒淀粉分支酶基因mRNA表达量随籽粒直链淀粉含量的变化发生变化,且能超亲表达;直链淀粉含量和淀粉分支酶活性高的基因型其SBE1和SBE3的mRNA表达量也高,低的基因型其mRNA表达量也低, SBE4的表达量与此相反.
選用籽粒直鏈澱粉含量有顯著差異的親本和雜種後代,分析籽粒灌漿過程中直鏈澱粉積纍和澱粉分支酶活性以及澱粉分支酶基因mRNA錶達量等變化特點.結果錶明,直鏈澱粉含量高的親本及後代在灌漿過程中閤成和積纍的直鏈澱粉量顯著高于直鏈澱粉含量低的親本及後代;直鏈澱粉含量高的親本及後代的澱粉分支酶活性均比直鏈澱粉含量低的親本及後代高,灌漿不同時期的籽粒直鏈澱粉含量與澱粉分支酶活性間均呈正相關;親本及雜種後代籽粒OsSBE1和OsSBE3的mRNA錶達量隨灌漿進程逐漸增加,達到峰值後又逐漸下降,呈單峰麯線變化,其錶達量峰值都齣現在抽穗後17 d,在灌漿過程中胚乳OsSBE4基因的mRNA錶達量相對比較小;雜種後代籽粒澱粉分支酶基因mRNA錶達量隨籽粒直鏈澱粉含量的變化髮生變化,且能超親錶達;直鏈澱粉含量和澱粉分支酶活性高的基因型其SBE1和SBE3的mRNA錶達量也高,低的基因型其mRNA錶達量也低, SBE4的錶達量與此相反.
선용자립직련정분함량유현저차이적친본화잡충후대,분석자립관장과정중직련정분적루화정분분지매활성이급정분분지매기인mRNA표체량등변화특점.결과표명,직련정분함량고적친본급후대재관장과정중합성화적루적직련정분량현저고우직련정분함량저적친본급후대;직련정분함량고적친본급후대적정분분지매활성균비직련정분함량저적친본급후대고,관장불동시기적자립직련정분함량여정분분지매활성간균정정상관;친본급잡충후대자립OsSBE1화OsSBE3적mRNA표체량수관장진정축점증가,체도봉치후우축점하강,정단봉곡선변화,기표체량봉치도출현재추수후17 d,재관장과정중배유OsSBE4기인적mRNA표체량상대비교소;잡충후대자립정분분지매기인mRNA표체량수자립직련정분함량적변화발생변화,차능초친표체;직련정분함량화정분분지매활성고적기인형기SBE1화SBE3적mRNA표체량야고,저적기인형기mRNA표체량야저, SBE4적표체량여차상반.
In this study, the amylase content which had significant difference in parents and hybrid progeny were used to analyze the characteristics of changes in the SBE gene relative expression, the accumulated amylose content and enzymes activities during grain filling. The results showed that the accumulated amylose content and enzymes activities in parents and hybrid progeny with high amylose contents were significantly higher than the low. The amylose content were positively correlated with enzymes activities during grain filling; the relative expression level of OsSBE1 and OsSBE3 activities increased to a peak and declined during grain filling with a single-peak curve. The expression peak appeared 17 d after heading. The expression of OsSBE4 was relatively small. The progeny with relative expression level of SBE gene changed with the change of the amylose content, and transgressive expression;the high genotypes of amylose content and enzymes activities increased with the relative expression level of OsSBE1 and OsSBE3, the low genotypes also had the low expression. The expression of OsSBE4 was to the contrary.
