CAJ | 학술논문

非特异性脂质转移蛋白(nsLTP)是植物中大量存在的小分子脂类结合蛋白,具有抗菌防御功能.分析黄瓜非特异性脂质转运蛋白基因nsLTP的序列特征及其在细菌性角斑病菌浸染过程中的表达模式,对其应用于黄瓜等农作物转基因工程,增强作物抗病性具有重要意义.在黄瓜叶cDNA文库中,获得非特异性脂质转移蛋白(nsLTP)cDNA序列,命名为CsnsLTP;该基因全长566 bp,编码121个氨基酸残基;序列分析表明,该基因具有N端信号肽,具有nsLTP家族共有的典型保守区域,属nsLTP家族基因.实时荧光定量PCR分析表明,CsnsLTP在黄瓜叶中有表达.在黄瓜细菌性角斑病菌浸染下,该基因在黄瓜叶中表达增高,并随浸染时间的延长而增强,明显受黄瓜细菌性角斑病菌的诱导,推测该基因在抵御黄瓜细菌性角斑病菌浸染时具有重要作用.
비특이성지질전이단백(nsLTP)시식물중대량존재적소분자지류결합단백,구유항균방어공능.분석황과비특이성지질전운단백기인nsLTP적서렬특정급기재세균성각반병균침염과정중적표체모식,대기응용우황과등농작물전기인공정,증강작물항병성구유중요의의.재황과협cDNA문고중,획득비특이성지질전이단백(nsLTP)cDNA서렬,명명위CsnsLTP;해기인전장566 bp,편마121개안기산잔기;서렬분석표명,해기인구유N단신호태,구유nsLTP가족공유적전형보수구역,속nsLTP가족기인.실시형광정량PCR분석표명,CsnsLTP재황과협중유표체.재황과세균성각반병균침염하,해기인재황과협중표체증고,병수침염시간적연장이증강,명현수황과세균성각반병균적유도,추측해기인재저어황과세균성각반병균침염시구유중요작용.
Plant non-specific lipid transfer proteins (nsLTPs) are abundantlipid binding proteins with small molecules that play several biological roles including antimicrobial defense. Analyzing the sequence characteristics of non-specific lipid transfer protein genes and their expression patterns challenged by Pseudomonas syringae pv. Lachrymans, which provides necessary data for further investigation of the crops transgene project and disease. resistance of cucumber. The cDNA sequence of the cucumber non-specific lipid transfer protein was obtained from the cucumber leaf cDNA library, termed as CsnsLTP; The length of CsnsLTP cDNA is 566 bp encoding 121 amino acid. sequence analysis revealed that the gene has the N terminal signal peptide and a typical nsLTP family conserved region, revealed the CsnsLTP belongs to nsLTP family gene. The expression analyses of the gene by real-time fluorescence quantitative PCR showed that the CsnsLTP express in the cucumber leaves. Challenged by Pseudomonas syringae pv. Lachrymans, the expression of the gene in cucumber leaves significantly increased with the extension of challenging time, which indicated that the CsnsLTP is induced by the challenging and might play an important role against the invasion of bacterium.