CAJ | 학술논문

将分离自内蒙古农家酸马奶(Koumiss)的粪肠球菌KLDS 4.1004经NTG诱变获得诱变株KLDS 4.1003,后者的LDH、DR酶活力下降,且ALD酶失活.对两者进行8个pH点条件下的酶活、生长情况与代谢物测定,确定KLDS 4.1003在pH 6.5、7.0时双乙酰产量最高.在6个时间点分别检测两个pH点的菌数与双乙酰产量变化,发现发酵16 h两项指标均最高,最终确定最高产双乙酰条件为pH 7.0、16 h,最大产量为1.29 g·L-1.结果表明,诱变株KLDS 4.1003产双乙酰性能优良,可进一步开发成产香型益生发酵剂菌种.
장분리자내몽고농가산마내(Koumiss)적분장구균KLDS 4.1004경NTG유변획득유변주KLDS 4.1003,후자적LDH、DR매활력하강,차ALD매실활.대량자진행8개pH점조건하적매활、생장정황여대사물측정,학정KLDS 4.1003재pH 6.5、7.0시쌍을선산량최고.재6개시간점분별검측량개pH점적균수여쌍을선산량변화,발현발효16 h량항지표균최고,최종학정최고산쌍을선조건위pH 7.0、16 h,최대산량위1.29 g·L-1.결과표명,유변주KLDS 4.1003산쌍을선성능우량,가진일보개발성산향형익생발효제균충.
A mutant strain KLDS 4.1003 was obtained via NTG from an Enterococcus faecalis KLDS 4.1004 which is separated from the Koumiss of Inner Mongolia. The activities of enzyme LDH and DR in KLDS 4.1003 decreased, while that of ALD was inactivated. The enzyme activities, growth statuses and metabolites of KLDS 4.1003 and KLDS 4.1004 at eight pH were determined. The yield of diacetyl in KLDS 4.1003 was the highest at pH 6.5 and 7.0, especially fermenting for 16 h. Therefore, the optimal condition of diacetyl production was at pH 7.0 for 16 h, and the highest yield was 1.29 g·L-1. The results showed that, for mutant strain KLDS 4.1003, the ability to produce diacetyl was excellent, and hence, it would be developed to an probiotic starters.