CAJ | 학술논문

以哈尔滨某气化厂生化车间活性污泥为菌源,在不同浓度间甲苯酚的选择培养基上培养,分离筛选后进行特性研究,并利用分子鉴定的方法对菌种进行鉴定.结果表明:当间甲苯酚含量为1000 mg·L-1,得到对间甲苯酚耐受能力最强的2个菌株,命名为JD-1和JD-2,根据间甲苯酚含量分别为300、500、600 mg·L-1的降解试验对比结果,确定JD-2菌为间甲苯酚优势降解菌.同时可知最优处理条件为:温度30℃,pH 7,葡萄糖量500 mg·L-1.通过对其菌落特征、生理生化性质以及16S rRNA测序鉴定结果得间甲苯酚优势降解菌JD-2属假单胞菌属(Pseudomonas sp.).
이합이빈모기화엄생화차간활성오니위균원,재불동농도간갑분분적선택배양기상배양,분리사선후진행특성연구,병이용분자감정적방법대균충진행감정.결과표명:당간갑분분함량위1000 mg·L-1,득도대간갑분분내수능력최강적2개균주,명명위JD-1화JD-2,근거간갑분분함량분별위300、500、600 mg·L-1적강해시험대비결과,학정JD-2균위간갑분분우세강해균.동시가지최우처리조건위:온도30℃,pH 7,포도당량500 mg·L-1.통과대기균락특정、생리생화성질이급16S rRNA측서감정결과득간갑분분우세강해균JD-2속가단포균속(Pseudomonas sp.).
Activated sludge of a gasification plant in Harbin was acted as biological bacteria source, different concentrations of m-cresol was selected as culture medium, studied characteristics after screening, moreover, molecular method was used as bacteria species identification. The results showed that, when the cresol content was 1 000 mg·L-1, it got 2 strains of the most m-cresol tolerance ability, named as the JD-1 and JD-2, and when cresol content was 300, 500, 600 mg·L-1; the degradation results was compared to determine JD-2 as Methyl-dominant bacteria. The optimal conditions were temperature 30℃ and pH 7, and the glucose content 500 mg·L-1 for JD-2.The results obtained by colony characteristics, physiological and biochemical properties and the 16S rRNA sequencing cresol dominant degradation bacteria was Pseudomonas sp. JD-2.