CAJ | 학술논문

目的应用VG染色对肝活检和尸检标本进行观察.探讨胶原纤维在慢性肝病组织内的分布及肝纤维化的形成机制.方法应用VG染色对40例肝活检和尸检标本进行检测,HE染色确定病理诊断.光镜下观察,VG染色显示慢性肝病组织中的胶原纤维.结果9例慢性肝炎中,轻型慢性肝炎2例,慢性中度肝炎4例,重度慢性肝炎3例;31例肝硬化中不完全分割性型3例,细结节型17例,粗结节型11例.经VG染色后胶原纤维染成粉红色,并形成间隔分割肝组织.结论胶原纤维可能起源于贮脂细胞和成纤维细胞,VG染色对慢性肝病有诊断意义.
목적응용VG염색대간활검화시검표본진행관찰.탐토효원섬유재만성간병조직내적분포급간섬유화적형성궤제.방법응용VG염색대40례간활검화시검표본진행검측,HE염색학정병리진단.광경하관찰,VG염색현시만성간병조직중적효원섬유.결과9례만성간염중,경형만성간염2례,만성중도간염4례,중도만성간염3례;31례간경화중불완전분할성형3례,세결절형17례,조결절형11례.경VG염색후효원섬유염성분홍색,병형성간격분할간조직.결론효원섬유가능기원우저지세포화성섬유세포,VG염색대만성간병유진단의의.
Objective To explore the distribution of collagen fiber in chronic liver disease tissue and hepatic fibrosis formation mechanism, VG staining was used to detect liver biopsy and autopsy specimens. Methods VG staining was used to detect 40 cases of liver biopsy and autopsy specimens, and HE staining was used to determine pathological diagnosis. VG staining showed the collagen fibrils of chronic liver diseases tissue under light microscope. Results Chronic hepatitis cases were 9, and low grade chronic hepatitis 2, moderate chronic hepatitis 4, serious chronic hepatitis 3;liver cirrhosis cases were 31,and incomplete partition type liver cirrhosis3, fine nodular cirrhosis 17, coarse nodular cirrhosis 11. By VG staining collagen fibers were dyed pink, and the form hepatic tissue interval. Conclusion Collagen fiber may originate in fat storing cells and fibroblasts. VG stains may have diagnostic significance in chronic liver disease.