CAJ | 학술논문

目的研究核转录因子-κB(NF-κB)及p53在布加综合征(BCS)合并肝细胞癌(HCC)中的作用及其机制.方法用免疫组织化学染色法检测NF-κB基因及p53基因在8例BCS合并HCC患者肝癌组织及其对应的癌旁肝组织的蛋白定位.结果 NF-κB蛋白在肝癌组织和癌旁肝组织的表达率分别为87.5%(7/8)和25.0%(2/8),差异有统计学意义(P<0.01),p53蛋白阳性在肝癌组织和癌旁肝组织的表达率分别为75.0%(6/8)和0(0/8),差异有统计学意义(P<0.01).结论 NF-κB及p53在肝癌组织中明显高表达,提示其可能参与BCS合并HCC的发生发展过程.NF-κB表达定位在癌组织和癌旁肝组织中的差异,提示NF-κB活化后,进入胞核,通过调节下游基因的转录,促进肝癌的发生,p53基因可能通过调控NF-κB信号通路活性参与肝癌的发生发展过程.
목적연구핵전록인자-κB(NF-κB)급p53재포가종합정(BCS)합병간세포암(HCC)중적작용급기궤제.방법용면역조직화학염색법검측NF-κB기인급p53기인재8례BCS합병HCC환자간암조직급기대응적암방간조직적단백정위.결과 NF-κB단백재간암조직화암방간조직적표체솔분별위87.5%(7/8)화25.0%(2/8),차이유통계학의의(P<0.01),p53단백양성재간암조직화암방간조직적표체솔분별위75.0%(6/8)화0(0/8),차이유통계학의의(P<0.01).결론 NF-κB급p53재간암조직중명현고표체,제시기가능삼여BCS합병HCC적발생발전과정.NF-κB표체정위재암조직화암방간조직중적차이,제시NF-κB활화후,진입포핵,통과조절하유기인적전록,촉진간암적발생,p53기인가능통과조공NF-κB신호통로활성삼여간암적발생발전과정.
Objective To investigate the correlations of expression of nuclear factor-kappa B (NF-κB) and mutant p53 in budd-chiari syndrome (BCS) with hepatocellular carcinoma (HCC). Methods 8 patients with BCS and HCC were composed and all the tissue sections included both HCC and surrounding non-tumor liver tissues. Expression of NF-κB and mutant p53 was examined using SP immunohistochemical technique. Results 7 patients (87.5%) showed decidedly positive staining of NF-κB in tumor liver tissues. While, only 2 patients (25.0%) of non-tumor Liver tissues were detected positive staining of NF-κB, there is a significant difference (P<0.01). No positive staining of p53 was detected in surrounding non-tumor liver tissues for all 8 patients. However, 6 patients showed positive staining of p53 (nucleon) there was a significant difference (P<0.01). Conclusion NF-κB gene is significantly overexpress and activated in liver cancer tissue, which may consequently modulate transcription of some related downstream-gene and promotes the initiation and development of BCS complicated with hepatocellular carcinoma, the p53 gene may be through the regulation of NF-κB signal pathway activity in the involvement and development of liver cancer.