CAJ | 학술논문

选用一株硅酸盐细菌,采用摇瓶发酵培养,对比分析不同结构硅酸盐矿物刺激与促进细菌产代谢产物的差异;采用摇瓶浸矿试验,研究细菌代谢产物及细菌对石英、斜长石与高岭石的风化分解作用.结果表明,硅酸盐矿物可促进细菌产草酸、酒石酸、苹果酸等有机酸、氨基酸与多糖,同时可刺激细菌代谢产生特定的柠檬酸与氨基酸,高岭石对细菌代谢产有机酸与多糖促进作用最强,斜长石对细菌代谢产生氨基酸促进作用最明显;各代谢产物均对硅酸盐矿物具有一定的风化分解作用,其中多糖对矿物分解作用最强,混合代谢产物对矿物具有更强的风化作用;具层状结构的高岭石较架状结构的斜长石更易被细菌及代谢产物所风化分解;3种代谢产物的混合物在风化矿物过程中具有明显协同作用;细菌对矿物分解作用明显较混合代谢产物强,表明细菌对矿物风化分解作用是生物物理及化学等多种因素共同作用的结果.
선용일주규산염세균,채용요병발효배양,대비분석불동결구규산염광물자격여촉진세균산대사산물적차이;채용요병침광시험,연구세균대사산물급세균대석영、사장석여고령석적풍화분해작용.결과표명,규산염광물가촉진세균산초산、주석산、평과산등유궤산、안기산여다당,동시가자격세균대사산생특정적저몽산여안기산,고령석대세균대사산유궤산여다당촉진작용최강,사장석대세균대사산생안기산촉진작용최명현;각대사산물균대규산염광물구유일정적풍화분해작용,기중다당대광물분해작용최강,혼합대사산물대광물구유경강적풍화작용;구층상결구적고령석교가상결구적사장석경역피세균급대사산물소풍화분해;3충대사산물적혼합물재풍화광물과정중구유명현협동작용;세균대광물분해작용명현교혼합대사산물강,표명세균대광물풍화분해작용시생물물리급화학등다충인소공동작용적결과.
The difference of bacterial metabolites secreted by the selected silicate bacterium in differrent media containing silicate minerals with different structures was investigated in shake flasks. The weathering of quartz, orthoclase and kaolinite by microbial metabolites and bacteria was researched by shake flask bioleaching experiments. The results show the tested silicate minerals can promote the strain to secrete organic acids (oxalate, tartaric acid and malic acid), amino acids and polysaccharide, and stimulate the strain to produce citric acid and some specific amino acids. Kaolinite promotes the strain to secrete organic acids and polysaccharide more obviously, and orthoclase stimulates the strain to produce amino acids higher effectively than other tested silicate minerals. Amino acid mixture, organic acid mixture and polysaccharide can weather tested silicate minerals, and polysaccharide has the strongest mineral weathering ability. The complex of all metabolites can decompose minerals more effectively than amino acid mixture, organic acid mixture and polysaccharide alone. Different metabolites have a certain synergistic effect on weathering anorthose. Kaolinite with layer structure are more easily decomposed than orthoclase with framework structure by bacteria. Bacteria can more effectively weather minerals in comparison with the metabolite complex, the result shows that silicate bacteria can weather silicate minerals at the integrated operation of many microbial, physical and chemical factors.