CAJ | 학술논문

目的本研究加工了一种封接有多孔聚碳酸酯薄膜的过滤式微流控芯片用于循环肿瘤细胞分选.方法依据肿瘤细胞与血细胞在粒径和变形能力方面的差别,这种微过滤式芯片可以选择性截留肿瘤细胞.循环肿瘤细胞分析整个过程,包括细胞过滤、固定、标记和计数,均在芯片上完成.使用8μm孔径滤膜,可以选择性截留Hela细胞.以Hela细胞为模型,实验优化了肿瘤细胞的分选条件并考查了分选效果.结果在500μL/min流速下,Hela细胞的回收率可以达到85%,而血细胞残留可以控制在3000个以下.截留细胞使用微小染色体维持蛋白7抗体标记,可以有效辨别肿瘤细胞与血细胞.结论基于过滤式微流控芯片上的循环肿瘤细胞分选方法简单、快速、廉价,有望成为一种实用的循环肿瘤细胞检测技术.
목적본연구가공료일충봉접유다공취탄산지박막적과려식미류공심편용우순배종류세포분선.방법의거종류세포여혈세포재립경화변형능력방면적차별,저충미과려식심편가이선택성절류종류세포.순배종류세포분석정개과정,포괄세포과려、고정、표기화계수,균재심편상완성.사용8μm공경려막,가이선택성절류Hela세포.이Hela세포위모형,실험우화료종류세포적분선조건병고사료분선효과.결과재500μL/min류속하,Hela세포적회수솔가이체도85%,이혈세포잔류가이공제재3000개이하.절류세포사용미소염색체유지단백7항체표기,가이유효변별종류세포여혈세포.결론기우과려식미류공심편상적순배종류세포분선방법간단、쾌속、렴개,유망성위일충실용적순배종류세포검측기술.
[ABSTRACT] Objective We fabricated a microfluidic device with embedded porous film for circulating tumor cell (CTC) enrichment from blood. Methods The microfilter can separate tumor cells from whole blood on the basis of differences in the size and deformability between tumor and hematologic cells. All the steps related to CTC analysis, including cell filtration, fixation, labeling and enumeration, were integrated on the same device. Our results demonstrated that Hela cells can specifically trapped on the porous film with 8μm pore-size. Using Hela cell as a model, operation conditions of the microfilter assay were optimized. Results At 500 μL/min flow rate, the capturing efficiency of Hela cell was 85%and the number of unspecific trapped hematologic cells can be limited to~3000 per test. Conclusions Using minichromosome maintenance protein 7 as the CTC characterization marker, the trapped Hela cells can be distinguished from the background blood cells. The microfilter-based CTC detection assay proposed here is simple, fast and low-cost, thus hold the potential to be developed into a practical CTC detection tool.