CAJ | 학술논문

慢病毒载体是一类逆转录病毒载体,以其转染效率高,可感染分裂期和非分裂期细胞,可容纳较大的基因片段等优点,现已被作为转移目的基因的理想载体.而RNA干扰技术是近年来迅速发展起来可以特异性地剔除特定基因或者抑制特定基因表达的一种新技术,在病毒感染、心血管疾病、肿瘤等疾病的治疗中被广泛应用.通过慢病毒载体与RNA干扰技术的结合应用,有望为特定基因的功能以及相关疾病的治疗研究提供新的手段和方法.
만병독재체시일류역전록병독재체,이기전염효솔고,가감염분렬기화비분렬기세포,가용납교대적기인편단등우점,현이피작위전이목적기인적이상재체.이RNA간우기술시근년래신속발전기래가이특이성지척제특정기인혹자억제특정기인표체적일충신기술,재병독감염、심혈관질병、종류등질병적치료중피엄범응용.통과만병독재체여RNA간우기술적결합응용,유망위특정기인적공능이급상관질병적치료연구제공신적수단화방법.
Lentiviral vector, a kind of retroviral vectors, has become an ideal vector for target gene transferation because of its high efficiency of transfection, the ability of transfection to the dividing or non-dividing cells and a capacity of large target gene fragments. RNA interference technology is a new kind of technology developing rapidly in recent years which can be used in eliminating a specific gene or suppressing the expression of a specific gene. And now it is widely applied in the treatment of virus infection, cardiovascular diseases, tumor and other diseases. With the combination of the lentiviral vectors and the RNA interference technology, it is expected to provide new methods for the study in the function of specific gene and the treatment of related diseases.