CAJ | 학술논문

从粳稻品种“嘉花1号”60Coγ射线辐照的后代中筛选到一个水稻温敏感失绿突变体tcm12,在20℃条件下的该突变体第2、3幼叶表现为失绿,第4叶开始完全转绿.而24℃以上条件其表型与野生型嘉花1号一致,呈正常绿色,表明其幼苗叶色表现出失绿是一种温度敏感性.透射电镜观察结果表明,突变体叶绿体在20℃条件下发育不全,而在32℃条件下正常发育.遗传分析表明,突变体的温度敏感失绿性状受1对隐性核基因(tcm12)控制,利用SSR和InDel分子标记以及广占63S/tcm12后代中76株F2突变型植株,将tcm12基因初定位在水稻第12号染色体长臂上的RM519和ID22406之间,然后,进一步利用目标区域内分子标记多态性较好的9311/tcm12组合的F2中分离出的157株突变型植株,最终将tcm12基因锁定在分子标记ID21199和ID21436之间的237 kb内.本研究结果为今后对该基因的克隆和功能分析奠定了基础.
종갱도품충“가화1호”60Coγ사선복조적후대중사선도일개수도온민감실록돌변체tcm12,재20℃조건하적해돌변체제2、3유협표현위실록,제4협개시완전전록.이24℃이상조건기표형여야생형가화1호일치,정정상록색,표명기유묘협색표현출실록시일충온도민감성.투사전경관찰결과표명,돌변체협록체재20℃조건하발육불전,이재32℃조건하정상발육.유전분석표명,돌변체적온도민감실록성상수1대은성핵기인(tcm12)공제,이용SSR화InDel분자표기이급엄점63S/tcm12후대중76주F2돌변형식주,장tcm12기인초정위재수도제12호염색체장비상적RM519화ID22406지간,연후,진일보이용목표구역내분자표기다태성교호적9311/tcm12조합적F2중분리출적157주돌변형식주,최종장tcm12기인쇄정재분자표기ID21199화ID21436지간적237 kb내.본연구결과위금후대해기인적극륭화공능분석전정료기출.
A thermo-sensitive chlorosis mutant, tcm12, derived from a progeny of a japonica rice variety, Jiahua No.1, treated by 60Co γ-radiation, displayed cholosis in the seedling of the 2nd and 3rd leaf stages under the low temperature below at 20℃, then turned normal green in the beginning of the 4th leaf growing Whereas the mutant can grow normally as the wild type variety, Jiahua No.1, at above 24℃, indicating that the trait of of chlorosis should be the thermo-sensitive. The transmission electron microscope assay showed that the chloroplast in the mutant was undeveloped under the temperature at 20℃below, but was well-developed under the condition of at 32℃. Then genetic analysis revealed that mutant trait was controlled by a single recessive nuclear gene (tcm12). Using SSR and InDel markers and 76 mutant-type F2 individuals from the population derived from the cross of Guangzhan63S/tcm12, tcm12 was roughly located between RM519 and ID22406 on the long arm of chromosome 12. Furthermore, 157 mutant-type F2 individuals from another population derived from the cross of 9311/tcm12 in view of better polymorphic markers on the target region, tcm12 was finally narrowed to in the range of 237 kb in length between the molecular markers of ID21199 and ID21436. The results obtained from the study may lay the basis for further cloning and functional analysis of this gene in rice.