CAJ | 학술논문

在克隆到水稻转录因子基因SUSIRI的基础上,构建其过表达及RNA干扰载体进行水稻的遗传转化.由潮霉素筛选及分子检测得到的T0代植株经大田栽植,共有34株过表达及46株RNA干扰植株获得T1代转基因种子.继续种植T1植株收获T2代种子,同时用半定量RT-PCR分析苗期及穗期野生型水稻中SUSIRI基因的时空表达谱并比较过表达及RNA干扰植株的SUSIRI基因在叶片及幼穗中的表达状况,发现过表达植株与野生型对照的基因表达基本相同,但RNA干扰植株的表达受到明显抑制.对过表达及RNA干扰植株稻穗性状考察表明:与野生型对照相比,无论是过表达还是RNA干扰,转基因植株的穗粒数、结实率降低,RNA干扰植株表现得尤为严重(P<0.05),但对水稻谷粒粒重的影响并不显著(P>0.05). SUSIRI基因的过表达未出现使稻穗性状明显改良的株系,而RNA干扰会导致水稻的结实能力严重降低.
재극륭도수도전록인자기인SUSIRI적기출상,구건기과표체급RNA간우재체진행수도적유전전화.유조매소사선급분자검측득도적T0대식주경대전재식,공유34주과표체급46주RNA간우식주획득T1대전기인충자.계속충식T1식주수획T2대충자,동시용반정량RT-PCR분석묘기급수기야생형수도중SUSIRI기인적시공표체보병비교과표체급RNA간우식주적SUSIRI기인재협편급유수중적표체상황,발현과표체식주여야생형대조적기인표체기본상동,단RNA간우식주적표체수도명현억제.대과표체급RNA간우식주도수성상고찰표명:여야생형대조상비,무론시과표체환시RNA간우,전기인식주적수립수、결실솔강저,RNA간우식주표현득우위엄중(P<0.05),단대수도곡립립중적영향병불현저(P>0.05). SUSIRI기인적과표체미출현사도수성상명현개량적주계,이RNA간우회도치수도적결실능력엄중강저.
Based on clonin g a transcriptional factor gene SUSIRI from rice, its transgenic vectors of both over-expression and RNA interference were constructed for rice transformation. Through hygromycin selection and molecular detection, T0 transgenic plants were grown to obtain the T1 seeds from 34 over-expressing plants and 46 RNAi plants. When T1 was planted for T2 harvest, the spatial and temporal expression of SUSIRI was analyzed with semi-quantitative RT-PCR in wild type rice and the expressing profile in leaf and panicle was compared with that of over-expressing and RNA interfering transgenic rice. It was found no difference in over-expressing plant, but the severe inhibition of SUSIRI expression occurred in RNA interfering transgenic rice. Investigation of panicle traits indicated that the number of grains per panicle and seed setting rate in all transgenic rice plant decreased as compared to wild type, and the difference at significant level (P<0.05) was frequent in RNA interfering transgenic rice. However, 1 000-grain weight among three varieties of rice had no significant difference (P>0.05). The over expression of SUSIRI gene had no obvious improvement of panicle traits in rice, but the RNA interference could result in the serious damage of seed-setting ability of rice.