CAJ | 학술논문

铵是植物吸收利用的主要氮源之一,NH4+的吸收主要通过铵转运蛋白(AMT)进行运输.本研究克隆了星星草铵转运蛋白(PutAMT),以GFP为标记构建了植物表达载体,观察表明PutAMT蛋白明显定位于质膜,但是根据观察发现PutAMT蛋白的定位具有复杂性及多样性的特点.为了深入了解铵转运蛋白的结构对NH4+吸收能力的影响,构建了N、C及NC末端缺失的PutAMT (ΔNPutAMT,ΔCPutAMT及ΔNCPutAMT)的载体,转入酵母突变体菌株31019b进行NH4+吸收能力的实验,结果显示了全长PutAMT在低氮条件下具有较强的吸收NH4+的功能.N、C及NC末端缺失的突变体与全长PutAMT相比,对NH4+的吸收能力有下降的趋势.结果表明了,N、C末端对NH4+的吸收起到重要的作用.
안시식물흡수이용적주요담원지일,NH4+적흡수주요통과안전운단백(AMT)진행운수.본연구극륭료성성초안전운단백(PutAMT),이GFP위표기구건료식물표체재체,관찰표명PutAMT단백명현정위우질막,단시근거관찰발현PutAMT단백적정위구유복잡성급다양성적특점.위료심입료해안전운단백적결구대NH4+흡수능력적영향,구건료N、C급NC말단결실적PutAMT (ΔNPutAMT,ΔCPutAMT급ΔNCPutAMT)적재체,전입효모돌변체균주31019b진행NH4+흡수능력적실험,결과현시료전장PutAMT재저담조건하구유교강적흡수NH4+적공능.N、C급NC말단결실적돌변체여전장PutAMT상비,대NH4+적흡수능력유하강적추세.결과표명료,N、C말단대NH4+적흡수기도중요적작용.
Ammonium is one of the important inorganic nitrogen source in plant absorption and utilization;the absorption of NH4+is mainly transported by the ammonium transporter (AMT). In this study, we cloned ammonium transporter (PutAMT) from Puccinellia tenuifolra. By means of the plant expression vector constructed with GFP marker, the observing results indicated that the PutAMT protein was mainly localized on the plasma membrane, however, according to the further observation we found the characteristics of PutAMT localization presented complexity and diversity. To further study how did the structure of ammonium transporter influence on the uptake capacity of NH4+, three truncated forms of PutAMT were constructed (ΔNPutAMT,ΔCPutAMT andΔNCPutAMT), and the constructed vectors were introduced into yeast mutant strain 31019b. The results showed that the full-length of PutAMT had the function of NH4+uptake under the condition of the low NH4+concentration, while three truncated forms of PutAMT (ΔNPutAMT,ΔCPutAMT andΔNCPutAMT) led to lost the capacity of NH4+uptake. The results suggested that N-or C-terminal should be both important for NH4+uptake.