CAJ | 학술논문

擎天凤梨的愈伤组织获取较为困难、而且愈伤分化缓慢且极易死亡,使用农杆菌介导转基因面临着受体难以获得、侵染后愈伤死亡率高、转化时间长以及成本高等问题.使用基因枪介导的转基因方法相对来说更为直接快速,但也面临转化效率不高、插入基因表达定位不清等问题.本研究以擎天凤梨组培苗的茎尖生长组织为材料,以基因枪介导结合GFP荧光蛋白基因,对转化中的主要影响因子进行研究,建立并优化了遗传转化体系,获得了转化再生植株,并利用GFP荧光蛋白基因的荧光特性,分析确定了外源基因的表达定位,为进一步利用分子育种手段培育擎天凤梨新品种打下基础.
경천봉리적유상조직획취교위곤난、이차유상분화완만차겁역사망,사용농간균개도전기인면림착수체난이획득、침염후유상사망솔고、전화시간장이급성본고등문제.사용기인창개도적전기인방법상대래설경위직접쾌속,단야면림전화효솔불고、삽입기인표체정위불청등문제.본연구이경천봉리조배묘적경첨생장조직위재료,이기인창개도결합GFP형광단백기인,대전화중적주요영향인자진행연구,건립병우화료유전전화체계,획득료전화재생식주,병이용GFP형광단백기인적형광특성,분석학정료외원기인적표체정위,위진일보이용분자육충수단배육경천봉리신품충타하기출.
It is known to hardly obtain the callus of Guzmania Bromeliad, and that the plant regenerates slowly with the high rate mortality, which make Agrobacterium-mediated genetic transformation of Guzmania Bromeliad more difficulties that face difficulty to obtaining explant, high mortality after infection, transformation time con-sumption and high costs. Particle bombardment-mediated genetic transformation is considered a more direct and quick way, but the problems of low transgenic efficiency and the unclear gene inserting sites exist. In this research we used top stem shoot of cultured seedling of Guzmania Bromeliad as material to study the main factors affecting genetic transformation, in order to establish and optimize the genetic transformation system of Guzmania Bro-meliad as well as obtain transgenic regenerated plant and detect and analyze the exogenous gene positioning and expression by using the Green fluorescent protein (GFP). The achievement of this research may lay the foundation to breed new Guzmania Bromeliad varieties by using molecular breeding approach.