CAJ | 학술논문

WRKY蛋白是植物中最大的转录因子家族之一,对植物的生长发育具有重要调控作用.本研究利用番茄全基因组测序结果鉴定了WRKY基因,分析了其系统发育关系,内含子-外显子结构,染色体上的分布及其表达方式.研究表明:番茄中存在81个WRKY转录因子,分为三类(Ⅰ,Ⅱ和Ⅲ),第Ⅰ和Ⅱ类分别细分为2和5个亚类,这些基因不均匀分布在番茄的11条染色体上;基因结构分析表明番茄WRKY转录因子进化过程中可能发生内含子的缺失/获得事件;不同芯片表达分析表明WRKY基因不仅参与了番茄根、子叶和真叶等不同组织类型的生长发育,而且还参与了一些生物胁迫(盐)和非生物胁迫(真菌激活子)的反应.
WRKY단백시식물중최대적전록인자가족지일,대식물적생장발육구유중요조공작용.본연구이용번가전기인조측서결과감정료WRKY기인,분석료기계통발육관계,내함자-외현자결구,염색체상적분포급기표체방식.연구표명:번가중존재81개WRKY전록인자,분위삼류(Ⅰ,Ⅱ화Ⅲ),제Ⅰ화Ⅱ류분별세분위2화5개아류,저사기인불균균분포재번가적11조염색체상;기인결구분석표명번가WRKY전록인자진화과정중가능발생내함자적결실/획득사건;불동심편표체분석표명WRKY기인불부삼여료번가근、자협화진협등불동조직류형적생장발육,이차환삼여료일사생물협박(염)화비생물협박(진균격활자)적반응.
Proteins of WRKY family, one of the largest families of transcription factors, play an important role in regulation of plant growth and development. In this research, we investigated tomato WRKY family genes based on the result of the whole genome sequencing and analyzed their phylogenetic relationships, the structure of introns and exons, the distribution of the chromosome as well as the model of expression. The result showed that 81 WRKY ranscription factors exists in the tomoto genome, which can be divided three types (Ⅰ,ⅡandⅢ), and TypeⅠ, SlWRKYⅠ, can be subdivided 2 sub-classes and TypeⅡ, SlWRKYⅡcan be subdivided 5 sub-classes;These genes unevenly distributes in 11 chromosomes of Tomoto. Analysis of the gene structure indicated that indel/acquire events of the intron may happen in the process of evolution of WRKY genes;Expression analysis of different chips revealed that SlWRKY not only involve in growth and development of different tissues such as root, cotyledon and leaf, but also response to biotic (salt) and abiotic (fungi activator) stresses.