分子植物育种
分子植物育種
분자식물육충
MOLECULAR PLANT BREEDING
2013年
1期
126-131
,共6页
骆菁菁*%李虹*%柏斌斌%俞红强 游捷**
駱菁菁*%李虹*%柏斌斌%俞紅彊 遊捷**
락정정*%리홍*%백빈빈%유홍강 유첩**
月季%月季品种‘光谱’%花青素%基因表达量
月季%月季品種‘光譜’%花青素%基因錶達量
월계%월계품충‘광보’%화청소%기인표체량
Rosa chinensis%Rose cultivar‘spectra’%Anthocyanin%Level of gene expression%qRT-PCR
探明变色月季花开放过程中花色变化的相关调控机制,可以为月季花色育种提供理论依据.本研究测定了月季‘光谱’在正常光照和遮光条件下的总花青素含量及月季‘光谱’花冠中CHS、DFR基因在两种条件下的差异表达,试图同时从代谢水平以及分子水平上进行探讨变色月季‘光谱’的变色机理.研究结果表明:光照是影响‘光谱’变色的关键环境因素,遮光阻碍了‘光谱’花瓣中花青素的合成,抑制了基因CHS和DFR的表达,导致花冠不着色,证明了花青素是决定月季的花冠由黄色变为红色的主要物质,也证明了基因CHS和DFR在花青素合成过程中的重要作用.
探明變色月季花開放過程中花色變化的相關調控機製,可以為月季花色育種提供理論依據.本研究測定瞭月季‘光譜’在正常光照和遮光條件下的總花青素含量及月季‘光譜’花冠中CHS、DFR基因在兩種條件下的差異錶達,試圖同時從代謝水平以及分子水平上進行探討變色月季‘光譜’的變色機理.研究結果錶明:光照是影響‘光譜’變色的關鍵環境因素,遮光阻礙瞭‘光譜’花瓣中花青素的閤成,抑製瞭基因CHS和DFR的錶達,導緻花冠不著色,證明瞭花青素是決定月季的花冠由黃色變為紅色的主要物質,也證明瞭基因CHS和DFR在花青素閤成過程中的重要作用.
탐명변색월계화개방과정중화색변화적상관조공궤제,가이위월계화색육충제공이론의거.본연구측정료월계‘광보’재정상광조화차광조건하적총화청소함량급월계‘광보’화관중CHS、DFR기인재량충조건하적차이표체,시도동시종대사수평이급분자수평상진행탐토변색월계‘광보’적변색궤리.연구결과표명:광조시영향‘광보’변색적관건배경인소,차광조애료‘광보’화판중화청소적합성,억제료기인CHS화DFR적표체,도치화관불착색,증명료화청소시결정월계적화관유황색변위홍색적주요물질,야증명료기인CHS화DFR재화청소합성과정중적중요작용.
Figuring out the possible regulatory mechanisms of flower color changing during the flowering stage of Chinese rosemight provide genetically insight for the color breeding of roses. This study was carried out at the metabolic and molecular level to measure total contents of anthocyanin and carotenoid as well as determine and the differential expression of CHS and DFR genes of the rose cultivar‘spectra’ under the light and shading conditions in order to explore the regulatory mechanism at the metabolic level and molecular level. The results showed that lighting was the key to effect the color change of rose cultivar‘spectra’. The shading condition hinders the synthesis of anthocyanin in the petals of‘spectra’, as well as inhibits the expression of genes CHS and DFR, which caused that the corolla couldn't colored. These results proved that the genes CHS and DFR play important roles in the process of anthocyanin synthesis, and also proved that anthocyanine is one of the factors that may determine the color changes of rose petals from yellow to red.
