CAJ | 학술논문

目的体外研究中药桂皮醛对口腔鳞状细胞癌细胞增殖的抑制作用.方法以3种人口腔鳞状细胞癌细胞株为研究对象,采用噻唑蓝(MTT)比色法观察桂皮醛不同质量浓度、不同作用时间下对各细胞株增殖的影响.应用 SPSS 16.0软件对数据进行方差分析.结果与空白对照组相比,256、128、64、32 mg·L-1质量浓度组的桂皮醛对人口腔鳞状细胞癌细胞的增殖表现出明显抑制作用,且抑制作用随着药物质量浓度的升高而加强,呈现明显的剂量-效应关系.药物作用时间24 h 时,作用质量浓度16 mg·L-1时,对人舌鳞状细胞癌 Tca8113细胞株抑瘤率达到58.33%;作用质量浓度为32 mg·L-1时对 KB 细胞及人颊黏膜鳞状细胞癌 BCaCD885细胞株的抑制率分别为66.08%、76.61%,其抑制率随着药物作用时间的延长而增加.结论桂皮醛对口腔鳞状细胞癌细胞增殖的抑制作用与质量浓度和作用时间有关,中、高浓度的桂皮醛对3种人口腔鳞状细胞癌细胞增殖均具有明显的抑制作用,在一定程度上肯定了桂皮醛的抗肿瘤作用.
목적체외연구중약계피철대구강린상세포암세포증식적억제작용.방법이3충인구강린상세포암세포주위연구대상,채용새서람(MTT)비색법관찰계피철불동질량농도、불동작용시간하대각세포주증식적영향.응용 SPSS 16.0연건대수거진행방차분석.결과여공백대조조상비,256、128、64、32 mg·L-1질량농도조적계피철대인구강린상세포암세포적증식표현출명현억제작용,차억제작용수착약물질량농도적승고이가강,정현명현적제량-효응관계.약물작용시간24 h 시,작용질량농도16 mg·L-1시,대인설린상세포암 Tca8113세포주억류솔체도58.33%;작용질량농도위32 mg·L-1시대 KB 세포급인협점막린상세포암 BCaCD885세포주적억제솔분별위66.08%、76.61%,기억제솔수착약물작용시간적연장이증가.결론계피철대구강린상세포암세포증식적억제작용여질량농도화작용시간유관,중、고농도적계피철대3충인구강린상세포암세포증식균구유명현적억제작용,재일정정도상긍정료계피철적항종류작용.
Objective To investigate the inhibiting in vitro effect of cinnamaldehyde on human oral squamous carcinoma cell. Methods Using methyl thiazolyl tetrazolium(MTT) to assay the effect of cinnamaldehyde on hu-man tongue carcinoma cell lines(Tca8113), human buccal squamous carcinoma cell lines(BCaCD885), human oral epithelial carcinoma KB cell in different concentrations. The final concentrations of cinnamaldehyde were from 8 to 256 mg·L-1, respectively. The cells were cultured in different concentrations, and the cinnamaldehyde continuously contacted with the cells for 24, 48 and 72 h, respectively, we compared the efficacy of cinnamaldehyde in dif-ferent concentration groups during different time quantum. Differences between data obtained were statistically analyzed using analysis of variance(ANOVA) in SPSS 16.0. Results When the concentration of cinnamicaldehyde were from 32 to 256 mg·L-1, it had a significant inhibition on the proliferation of oral cancer cells in vitro, and with a increasing concentration of cinnamicaldehyde, the efficacy of inhibition became more significant, which showed an obvious dosage-efficacy relationship. When cinnamaldehyde acted 24 hours or its concentration was 16 mg·L-1, the inhibition rate was 58.33% for the human tongue squamous cell carcinoma cell line Tca8113; when the concentration was on the level of 32 mg·L-1, the inhibition rate were 66.08%, 76.61% respectively for human oral epithelial carcinoma KB cell and human buccal squamous carcinoma cell BCaCD885, respectively. Conclusion In the medium-high(from 32 to 256 mg·L-1) concentrations of cinnamaldehyde showed remarkable cytostatic activity in terms of inhibition the proliferation of oral squamous carcinoma epithelial cell.