CAJ | 학술논문

宏基因组学研究特定生物环境中全部微小生物的基因组,直接从土壤和海水以及人体胃肠道和口腔等环境中获取样品 DNA,利用适宜的载体将其克隆到替代宿主细胞中构建宏基因文库,以筛选新的活性物质和新的基因;因此,利用宏基因组学技术不仅能够有效地检测口腔微生物群落结构,同时还极大地扩展了口腔微生物资源的利用空间,增加了获得新的生物活性物质和基因的机会.本文就宏基因组学的研究方法和宏基因组学口腔微生物研究领域中的应用等研究进展作一综述.
굉기인조학연구특정생물배경중전부미소생물적기인조,직접종토양화해수이급인체위장도화구강등배경중획취양품 DNA,이용괄의적재체장기극륭도체대숙주세포중구건굉기인문고,이사선신적활성물질화신적기인;인차,이용굉기인조학기술불부능구유효지검측구강미생물군락결구,동시환겁대지확전료구강미생물자원적이용공간,증가료획득신적생물활성물질화기인적궤회.본문취굉기인조학적연구방법화굉기인조학구강미생물연구영역중적응용등연구진전작일종술.
Metagenomics is the genomes of the total microbiota found in nature. Metagenome libraries were con-structed by directly extracting DNA from environmental samples, Such as soil, oceans, human gastrointestinal tract and mouth, etc., and transforming to surrogate host. The libraries were screened for novel bioactive compounds or genes. Therefore, Metagenomics can effectively detect oral microbial community structure, and also can enormously amplified the space of microbial resource utilization and enhanced the opportunity of obtain novel bioactive com-pounds. This review included the research progress of the methods of metagenomic and the application of metage-nomic study in the oral microbiota.