国际口腔医学杂志
國際口腔醫學雜誌
국제구강의학잡지
JOURNAL OF INTERNATIONAL STOMATOLOGY
2013年
2期
144-147
,共4页
非综合征性唇腭裂%蛋氨酸合成酶%亚甲基四氢叶酸脱氢酶1%基因多态性
非綜閤徵性脣腭裂%蛋氨痠閤成酶%亞甲基四氫葉痠脫氫酶1%基因多態性
비종합정성진악렬%단안산합성매%아갑기사경협산탈경매1%기인다태성
non-syndromic cleft lip with or without cleft palate%mehionine synthase%methylenetetrahydrofo-late dehydrogenase 1%single nucleotide polymorphism
目的研究亚甲基四氢叶酸脱氢酶(MTHFD)1和蛋氨酸合成酶(MTR)基因多态性与非综合征性唇腭裂(NSCL/P)发生的相关性.方法取187例NSCL/P患者裂隙处的黏膜组织为试验组,157例对照组的正常的口腔黏膜组织为对照组,检测MTHFD1基因(rs2236225位点)和MTR(rs1805087位点)基因型,比较两组的基因型和等位基因频率,分析基因型分布频率、两个基因的共同作用及其与NSCL/P的发生是否相关.结果 MTR、 MTHFD1在两组间的基因型和等位基因分布上的差异无统计学意义(P>0.05); MTR的AG、 GG基因型相对于AA基因型的比值比分别为:0.308、0.501. MTHFD1的GA、 AA基因型相对于GG基因型的比值比分别为:0.812、0.196. AG-AA型、 GG-GA型、 GG-AA型相对于AA-GG型的比值比分别是2.078、4.508、6.497.结论 MTHFD1和MTR的基因多态性均与NSCL/P的发生之间没有相关性,但是,它们之间相互作用是NSCL/P发生的危险因素.
目的研究亞甲基四氫葉痠脫氫酶(MTHFD)1和蛋氨痠閤成酶(MTR)基因多態性與非綜閤徵性脣腭裂(NSCL/P)髮生的相關性.方法取187例NSCL/P患者裂隙處的黏膜組織為試驗組,157例對照組的正常的口腔黏膜組織為對照組,檢測MTHFD1基因(rs2236225位點)和MTR(rs1805087位點)基因型,比較兩組的基因型和等位基因頻率,分析基因型分佈頻率、兩箇基因的共同作用及其與NSCL/P的髮生是否相關.結果 MTR、 MTHFD1在兩組間的基因型和等位基因分佈上的差異無統計學意義(P>0.05); MTR的AG、 GG基因型相對于AA基因型的比值比分彆為:0.308、0.501. MTHFD1的GA、 AA基因型相對于GG基因型的比值比分彆為:0.812、0.196. AG-AA型、 GG-GA型、 GG-AA型相對于AA-GG型的比值比分彆是2.078、4.508、6.497.結論 MTHFD1和MTR的基因多態性均與NSCL/P的髮生之間沒有相關性,但是,它們之間相互作用是NSCL/P髮生的危險因素.
목적연구아갑기사경협산탈경매(MTHFD)1화단안산합성매(MTR)기인다태성여비종합정성진악렬(NSCL/P)발생적상관성.방법취187례NSCL/P환자렬극처적점막조직위시험조,157례대조조적정상적구강점막조직위대조조,검측MTHFD1기인(rs2236225위점)화MTR(rs1805087위점)기인형,비교량조적기인형화등위기인빈솔,분석기인형분포빈솔、량개기인적공동작용급기여NSCL/P적발생시부상관.결과 MTR、 MTHFD1재량조간적기인형화등위기인분포상적차이무통계학의의(P>0.05); MTR적AG、 GG기인형상대우AA기인형적비치비분별위:0.308、0.501. MTHFD1적GA、 AA기인형상대우GG기인형적비치비분별위:0.812、0.196. AG-AA형、 GG-GA형、 GG-AA형상대우AA-GG형적비치비분별시2.078、4.508、6.497.결론 MTHFD1화MTR적기인다태성균여NSCL/P적발생지간몰유상관성,단시,타문지간상호작용시NSCL/P발생적위험인소.
Objective To investigate the association between genetic polymorphism of methylenetetrahydrofolate dehydrogenase(MTHFD)1 and mehionine synthas(MTR) and non-syndromic cleft lip with or without cleft palate (NSCL/P). Methods Collected the mucosa of the experimental group consisted of 187 NSCL/P patients and control group consisted of 157 normal mucosa. DNA was extracted and identified the genotypes of the samples:MTHFD1(rs2236225) and MTR(rs1805087). Compared with two groups of genotype and allele frequency, analysis of genotype distribution frequency and relationship between two genes’ interaction with NSCL/P. Results There was no statistical difference in genotype and gene frequencies between case group and control group of MTR and MTHFD1 (P>0.05). The odds ratio of genotype AG, GG of MTR were 0.308, 0.501. The odds ratio of genotype GA, AA of MTHFD1 were 0.812, 0.196. According to AA-GG, the odds ratio of genotype between AG-AA, GG-GA and GG-AA were 2.078, 4.508 and 6.497. Conclusion None association between genetic polymorphism of MTHFD1, MTR and NSCL/P, but the interaction of MTHFD1 and MTR is the risk factor of the NSCL/P.
