CAJ | 학술논문

采用逐量分批驯化方法,从某造纸厂的废水中分离得到一株能够以苯酚为唯一碳源生长、可高效降解苯酚的菌株 F5-2.通过形态学、生理生化特征和16S rDNA 序列分析,F5-2鉴定为节杆菌(Arthrobacter sp.).该菌株的细胞生长与苯酚降解能力呈同步趋势,苯酚降解主要发生在生长对数期.该菌株最高可耐受1500 mg?L?1的苯酚,在温度20~40℃、pH 5.0~9.0、摇床转速200 r?min?1、盐度0~40 g?L?1范围内,F5-2菌株均能保持对苯酚良好的降解能力.菌种接种量在2%时苯酚降解率达到最大;反应57 h 后,可使800 mg?L?1的苯酚降解率达96.13%.F5-2菌株降解苯酚的功能酶是邻苯二酚2,3-双加氧酶,该酶为胞内酶,其表达受苯酚诱导,通过间位开环降解苯酚.
채용축량분비순화방법,종모조지엄적폐수중분리득도일주능구이분분위유일탄원생장、가고효강해분분적균주 F5-2.통과형태학、생리생화특정화16S rDNA 서렬분석,F5-2감정위절간균(Arthrobacter sp.).해균주적세포생장여분분강해능력정동보추세,분분강해주요발생재생장대수기.해균주최고가내수1500 mg?L?1적분분,재온도20~40℃、pH 5.0~9.0、요상전속200 r?min?1、염도0~40 g?L?1범위내,F5-2균주균능보지대분분량호적강해능력.균충접충량재2%시분분강해솔체도최대;반응57 h 후,가사800 mg?L?1적분분강해솔체96.13%.F5-2균주강해분분적공능매시린분이분2,3-쌍가양매,해매위포내매,기표체수분분유도,통과간위개배강해분분.
A strain named F5-2 capable of utilizing phenol as the sole carbon source and with high efficiency of phenol degradation was isolated from the waste water of a paper mill plant by means of gradually increasing the concentration of phenol in the culture medium. According to its morphology, biochemical characteristics, and the 16S rDNA sequence, this strain was identified as Arthrobacter sp. The phenol degradation activity of this bacterial strain is proportional to its cell growth and the degradation occurs mainly during the logarithmic growth period. The highest phenol concentration that the strain could tolerate to grow is 1500 mg?L?1. In the temperature range of 20~40℃, pH 5.0~9.0, shaking rate of 200 r?min?1, and salinity of 0~40 g·L-1, strain F5-2 remains high phenol degradation activity, and 96.13% of the phenol can be degraded within 57 hours when the initial phenol concentration is 800 mg?L?1 and with a bacterial inoculum size of 2% of the culture medium. The functional enzyme responsible for the phenol degradation in strain F5-2 is the intracellularly localized catechol 2,3-dioxygenase, which is phenol inducible and can cleave the ortho-aromatic ring to degrade the phenol.