CAJ | 학술논문

内生拟盘多毛孢菌J63(Pestalotiopsis sp. J63)是从东海的海滩滩涂中新分离出的一株真菌.研究考察了无机盐对该菌株产漆酶响,以及对漆酶催化底物和脱色偶氮类染料甲基橙的影响.结果表明添加H3BO3、SrCl 2·6H2ONaF、KBr、MnCl2·4H2O和ZnSO4·7H2O对漆酶的产生有明显的促进作用,其中H3BO3的作用最明显,可使酶活增加221.8%.在漆酶催化底物反应时,除1 mmol?L?1 FeSO4·7H2O能够抑制漆酶酶活,其余无机盐均对酶活有促进作用.14 mmol?L?1 KAl(SO4)2对酶活的促进作用明显,使相对酶活达到124.9%.3 mmol?L?1 KBr使相对酶活达到116.7%,与其他菌株所产漆酶受到KBr抑制的特性相比具有优势.添加14 mmol?L?1 KAl(SO4)2和3 mmol?L?1 KBr后相应的脱色率分别达到了97.3%和75.6%,高于对照组64%的脱色率.同时还研究了适宜该菌株产漆酶的温度和pH,当温度为26 oC,pH为6.0时酶活最高,达到3280.6 U?L?1.
내생의반다모포균J63(Pestalotiopsis sp. J63)시종동해적해탄탄도중신분리출적일주진균.연구고찰료무궤염대해균주산칠매향,이급대칠매최화저물화탈색우담류염료갑기등적영향.결과표명첨가H3BO3、SrCl 2·6H2ONaF、KBr、MnCl2·4H2O화ZnSO4·7H2O대칠매적산생유명현적촉진작용,기중H3BO3적작용최명현,가사매활증가221.8%.재칠매최화저물반응시,제1 mmol?L?1 FeSO4·7H2O능구억제칠매매활,기여무궤염균대매활유촉진작용.14 mmol?L?1 KAl(SO4)2대매활적촉진작용명현,사상대매활체도124.9%.3 mmol?L?1 KBr사상대매활체도116.7%,여기타균주소산칠매수도KBr억제적특성상비구유우세.첨가14 mmol?L?1 KAl(SO4)2화3 mmol?L?1 KBr후상응적탈색솔분별체도료97.3%화75.6%,고우대조조64%적탈색솔.동시환연구료괄의해균주산칠매적온도화pH,당온도위26 oC,pH위6.0시매활최고,체도3280.6 U?L?1.
Pestalotiopsis sp. J63 is a fungus isolated from the oceanic sediment of the East China Sea. The effect of inorganic salts on laccase production, laccase activity and azo dye (methyl orange) decolorization was investigated in this work. The results suggest that the additions of H3BO3, SrCl2·6H2O, NaF, KBr, MnCl2·4H2O and ZnSO4·7H2O have the positive effect on laccase production. Among them, H3BO3 enhances laccase activity by 221.8% which is the most effective one. In the catalytic reaction process, all salts increase laccase activity except for FeSO4·7H2O of 1 mmol?L?1. Moreover, 14 mmol?L?1 KAl(SO4)2 and 3 mmol?L?1 KBr increase laccase activity to 124.9% and 116.7% in comparison with the control, respectively. Corresponding decolorization rates also enhance to 97.3%and 75.6%, which are much higher than that of the control sample (64%). Then, the appropriate temperature and pH for laccase formation were tested in fermentation, and it was found that 26℃ and pH 6.0 give the highest laccase activity (3280.6 U?L?1).