CAJ | 학술논문

在自主研发无蛋白培养基的基础上,考察了氨基酸、维生素和葡萄糖对 CHO 细胞生长、代谢与抗体合成的影响.结果表明,对培养过程中消耗较多的氨基酸进行补充,虽然不能促进细胞生长,但有利于培养后期细胞活性维持与抗体合成;B 族维生素的添加能促进细胞生长并延长培养时间;作为重要的碳源和能源,葡萄糖浓度较高时会抑制细胞生长,而浓度较低时不能有效支持细胞生长与抗体合成,维持其在适当浓度有利于细胞生长并能延长培养时间,从而有利于提高抗体产量.通过合理调整各营养物的浓度配比形成了优化的无蛋白培养基,CHO细胞在该培养基中的最高密度达到52.6×105 cells?mL?1,抗体产量达到274 mg?L?1,与初始培养基相比分别提高了33%和63%.总之,在细胞培养过程中应维持充足且平衡的营养物成分,以有效供应细胞生长与抗体合成的需求.
재자주연발무단백배양기적기출상,고찰료안기산、유생소화포도당대 CHO 세포생장、대사여항체합성적영향.결과표명,대배양과정중소모교다적안기산진행보충,수연불능촉진세포생장,단유리우배양후기세포활성유지여항체합성;B 족유생소적첨가능촉진세포생장병연장배양시간;작위중요적탄원화능원,포도당농도교고시회억제세포생장,이농도교저시불능유효지지세포생장여항체합성,유지기재괄당농도유리우세포생장병능연장배양시간,종이유리우제고항체산량.통과합리조정각영양물적농도배비형성료우화적무단백배양기,CHO세포재해배양기중적최고밀도체도52.6×105 cells?mL?1,항체산량체도274 mg?L?1,여초시배양기상비분별제고료33%화63%.총지,재세포배양과정중응유지충족차평형적영양물성분,이유효공응세포생장여항체합성적수구.
Based on a protein-free culture medium with independent intellectual property rights owned by our lab, the effects of amino acids, vitamins and glucose on cell growth, metabolism and antibody production were investigated. It was found that supplementation of those most consumed amino acids promotes cell viability and antibody production in the later stage, however the supplementation doesn’t promote the cell growth and the cell concentration remains unchanged. The reasonable addition of some B vitamins improves cell growth and extends culture duration. As an important carbon and energy source, high concentration of glucose inhibits cell growth, while low concentration will be inefficient to support the cell growth and antibody production. It was demonstrated that by maintaining the glucose concentration in an optimal range, cell growth is improved and culture duration is prolonged, which are favorable for antibody production. By optimizing the fractions of nutrient components in the basal culture medium, an optimal protein-free culture medium for CHO cells was developed. The maximum viable cell concentration and antibody productivity in that medium are 52.6×105 cells?mL?1 and 274 mg?L?1, respectively, which represent an increase of 33%and 63%in comparison with those in basal culture medium. In brief, the nutrients in culture medium should be sufficient and balanced for cell growth and antibody production.