CAJ | 학술논문

利用微通道结合结晶致孔方法制备了孔隙发达、粒径分布均匀的聚甲基丙烯酸羟乙酯(pHEMA)晶胶微球.晶胶微球平均粒径924.4μm,一致性多分散系数0.21.晶胶微球填装于直径10 mm层析柱内,填充高度91 mm;经甲基丙烯酸二甲氨乙酯(DMAEMA)修饰后应用于三磷酸腺苷(ATP)分离.层析过程流速为2 cm?min?1,以0.01 mol?L?1 HCl为缓冲液,分别用0.03 mol?L?1和1 mol?L?1 NaCl溶液进行洗脱.利用晶胶微球填充床从三组不同ATP含量转化液中可获得纯度达93.4%~96.9%的ATP,总回收率为47.0%~82.7%.
이용미통도결합결정치공방법제비료공극발체、립경분포균균적취갑기병희산간을지(pHEMA)정효미구.정효미구평균립경924.4μm,일치성다분산계수0.21.정효미구전장우직경10 mm층석주내,전충고도91 mm;경갑기병희산이갑안을지(DMAEMA)수식후응용우삼린산선감(ATP)분리.층석과정류속위2 cm?min?1,이0.01 mol?L?1 HCl위완충액,분별용0.03 mol?L?1화1 mol?L?1 NaCl용액진행세탈.이용정효미구전충상종삼조불동ATP함량전화액중가획득순도체93.4%~96.9%적ATP,총회수솔위47.0%~82.7%.
Poly(2-hydroxyethyl methacrylate) cryogel microbeads were prepared by combining the microchannel and cryo-polymerization methods under frozen condition. The mean diameter of the cryogel microbeads is 924.4 μm with a narrow particle size distribution and the consistency of multi-dispersion coefficient is 0.21. The prepared cryogel microbeads were packed in a chromatography column with diameter of 10 mm and packing height of 91 mm and then they were grafted with N,N-dimethylaminoethyl methacrylate (DMAEMA) monomer. The grafted cryogel microbead bed was used to isolate the adenosine triphosphate (ATP). Chromatographic separation of ATP from the fermentation broths was carried out at the flow velocity of 2 cm?min?1 with 0.01 mol?L?1 HCl as running buffer and 0.03 and 1 mol?L?1 NaCl solutions, respectively as eluting liquid. The high purity ATP was recovered from the Saccharomyces cerevisiae fermentation broth feedstocks containing different contents of ATP, adenosine diphosphate (ADP), adenosine monophosphate (AMP), other impurities and yeast cells. The purities and the recovery ratios of the obtained ATP are about 93.4%~96.9%and 47.0%~82.7%, respectively.