应用预防医学
應用預防醫學
응용예방의학
JOURNAL OF APPLIED PREVENTIVE MEDICINE
2012年
6期
321-325
,共5页
赵明明%胡才友%杨泽%吕泽平%孙亮%李俊纯%黄泽治%李正杰%韦勇%史晓红%梁庆华%吕渊
趙明明%鬍纔友%楊澤%呂澤平%孫亮%李俊純%黃澤治%李正傑%韋勇%史曉紅%樑慶華%呂淵
조명명%호재우%양택%려택평%손량%리준순%황택치%리정걸%위용%사효홍%량경화%려연
长寿%ApoE基因%永福县
長壽%ApoE基因%永福縣
장수%ApoE기인%영복현
Longevity%Apolipoprotein E gene%Yongfu County
目的研究广西永福县长寿老人载脂蛋白E(ApoE)基因多态性与永福长寿现象的关系.方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术,对319名年龄90~113岁的广西永福县长寿老人和168名作为相应年龄对照的长寿家庭成员,并以当地非长寿自然人群104人作为对照组,进行ApoE基因分型,并分别比较长寿老人与各组人群的基因型、基因频率的分布特征.结果(1)广西永福县长寿老人ApoE基因型频率中以ε3/3最高,其次是ε2/3和ε3/4,而ε2/4和ε4/4频率最少.长寿老人组与其亲属年龄对照的ApoE基因型和基因频率相比之间均无显著性差别.(2)长寿组与当地自然人群对照相比ε3/ε3(82.4%;75.0%)基因型和ε3等位基因(90.44%;85.17%)呈显著性阳性关联(P <0.05,P <0.01).结论 ApoE 基因多态性与广西永福县长寿现象有关联.ApoEε3等位基因与永福长寿有关联,ApoEε3/ε3纯合子是最主要的永福长寿关联基因.
目的研究廣西永福縣長壽老人載脂蛋白E(ApoE)基因多態性與永福長壽現象的關繫.方法採用聚閤酶鏈反應-限製性片段長度多態性(PCR-RFLP)技術,對319名年齡90~113歲的廣西永福縣長壽老人和168名作為相應年齡對照的長壽傢庭成員,併以噹地非長壽自然人群104人作為對照組,進行ApoE基因分型,併分彆比較長壽老人與各組人群的基因型、基因頻率的分佈特徵.結果(1)廣西永福縣長壽老人ApoE基因型頻率中以ε3/3最高,其次是ε2/3和ε3/4,而ε2/4和ε4/4頻率最少.長壽老人組與其親屬年齡對照的ApoE基因型和基因頻率相比之間均無顯著性差彆.(2)長壽組與噹地自然人群對照相比ε3/ε3(82.4%;75.0%)基因型和ε3等位基因(90.44%;85.17%)呈顯著性暘性關聯(P <0.05,P <0.01).結論 ApoE 基因多態性與廣西永福縣長壽現象有關聯.ApoEε3等位基因與永福長壽有關聯,ApoEε3/ε3純閤子是最主要的永福長壽關聯基因.
목적연구엄서영복현장수노인재지단백E(ApoE)기인다태성여영복장수현상적관계.방법채용취합매련반응-한제성편단장도다태성(PCR-RFLP)기술,대319명년령90~113세적엄서영복현장수노인화168명작위상응년령대조적장수가정성원,병이당지비장수자연인군104인작위대조조,진행ApoE기인분형,병분별비교장수노인여각조인군적기인형、기인빈솔적분포특정.결과(1)엄서영복현장수노인ApoE기인형빈솔중이ε3/3최고,기차시ε2/3화ε3/4,이ε2/4화ε4/4빈솔최소.장수노인조여기친속년령대조적ApoE기인형화기인빈솔상비지간균무현저성차별.(2)장수조여당지자연인군대조상비ε3/ε3(82.4%;75.0%)기인형화ε3등위기인(90.44%;85.17%)정현저성양성관련(P <0.05,P <0.01).결론 ApoE 기인다태성여엄서영복현장수현상유관련.ApoEε3등위기인여영복장수유관련,ApoEε3/ε3순합자시최주요적영복장수관련기인.
Objective To study the relationship between the long-lived apolipoprotein E (ApoE ) gene polymorphism and the longevity phenomenon in Yongfu County,Guangxi Province. Methods By polymerase chain reaction -restriction fragment length polymorphism ( PCR-RFLP ) technique ,319 longevity elderly aged 90 -113 years in Yongfu County and 168 aged matched control longevity elderly as a corresponding member of the family were selected and 104 local non longevity elderly in natural populations were selected as the control group,ApoE genotypes were determined; AproE genotypes and the characteristics of gene frequency distribution between the longevious group and other groups were also compared.Results (1) ApoE genotype frequency ε3 / 3 was the highest in the longevity elderly inYongfu County, followed by the ε2 / 3 and ε3 / 4,;the lowest frequencies from ε2 / 4 and 4/4 were observed. There was no significant difference in ApoE genotype and gene frequency between the longevity elderly group and family members. (2) The comparition between the longevity group and the local natural population showed a significant positive correlation (P <0.05,P <0.01) for the ε3/ε3 (82.4%;75.0%) genotype and ε 3 allele (90.44% ; 85.17% ).Conclusions ApoE gene polymorphism is associated with the phenomenon of longevity in Yongfu County,Guangxi. ApoE ε 3 allele is associated with Yongfu longevity,ApoE ε 3/ ε3homozygotes are perhaps the main Yongfu longevity related genes.
