应用预防医学
應用預防醫學
응용예방의학
JOURNAL OF APPLIED PREVENTIVE MEDICINE
2012年
6期
325-331
,共7页
唐振柱%李秀桂%孙贵娟%黄彦%黄兆勇%丁伟%龚祖康%王红%吕素玲%诸葛石养%韦程媛%方志峰
唐振柱%李秀桂%孫貴娟%黃彥%黃兆勇%丁偉%龔祖康%王紅%呂素玲%諸葛石養%韋程媛%方誌峰
당진주%리수계%손귀연%황언%황조용%정위%공조강%왕홍%려소령%제갈석양%위정원%방지봉
监测技术%大型活动%食品安全%效果
鑑測技術%大型活動%食品安全%效果
감측기술%대형활동%식품안전%효과
Monitoring technology%Large-scale activities in the provincial capital%Food safety%Effectiveness
目的建立“食品-餐饮-哨点医院”监测管理模式与重点食源性致病菌实时荧光PCR快速检验及溯源分析方法,并在保障大型活动食品安全活动中推广应用.方法采用“食品-餐饮-哨点医院”监测管理模式和实时荧光 PCR 快速方法开展对南宁市市售及大型活动中餐饮重点食品中致病菌和食源性病原株主动监测,应用PFGE分子分型方法开展溯源分析,结合创新餐饮管理模式,开展食品安全评估和预警.结果首府-南宁市市售食品及餐饮食品中均存在不同程度的副溶血性弧菌、沙门氏菌、志贺氏菌、肠出血性O157:H7、单核细胞增生李斯特氏菌、金黄色葡萄球菌、空肠弯曲菌、阪崎肠杆菌等致病菌污染,其中以副溶血性弧菌检出率最高达41.8%,其次是金黄色葡萄球菌为21.0%;腹泻标本中分离食源性致病菌与食用食品有直接关系;食源性致病菌危险评估与预警机制建立及大型活动食品安全管理模式的应用有效减少了食源性疾病发生.5年来,首府南宁市在大型活动期间未发生食物中毒事件,全市食物中毒起数呈逐年下降趋势;实时荧光PCR和基因指纹图谱检测方法能够快速检测食品和腹泻标本中食源性致病菌,通过散发性病例中的亲缘关系可溯源到原因食品.结论“食品-餐饮-哨点医院”监测管理新模式的应用及检测新方法的使用可有效预防和控制食源性疾病的发生,确保大型活动食品安全.
目的建立“食品-餐飲-哨點醫院”鑑測管理模式與重點食源性緻病菌實時熒光PCR快速檢驗及溯源分析方法,併在保障大型活動食品安全活動中推廣應用.方法採用“食品-餐飲-哨點醫院”鑑測管理模式和實時熒光 PCR 快速方法開展對南寧市市售及大型活動中餐飲重點食品中緻病菌和食源性病原株主動鑑測,應用PFGE分子分型方法開展溯源分析,結閤創新餐飲管理模式,開展食品安全評估和預警.結果首府-南寧市市售食品及餐飲食品中均存在不同程度的副溶血性弧菌、沙門氏菌、誌賀氏菌、腸齣血性O157:H7、單覈細胞增生李斯特氏菌、金黃色葡萄毬菌、空腸彎麯菌、阪崎腸桿菌等緻病菌汙染,其中以副溶血性弧菌檢齣率最高達41.8%,其次是金黃色葡萄毬菌為21.0%;腹瀉標本中分離食源性緻病菌與食用食品有直接關繫;食源性緻病菌危險評估與預警機製建立及大型活動食品安全管理模式的應用有效減少瞭食源性疾病髮生.5年來,首府南寧市在大型活動期間未髮生食物中毒事件,全市食物中毒起數呈逐年下降趨勢;實時熒光PCR和基因指紋圖譜檢測方法能夠快速檢測食品和腹瀉標本中食源性緻病菌,通過散髮性病例中的親緣關繫可溯源到原因食品.結論“食品-餐飲-哨點醫院”鑑測管理新模式的應用及檢測新方法的使用可有效預防和控製食源性疾病的髮生,確保大型活動食品安全.
목적건립“식품-찬음-초점의원”감측관리모식여중점식원성치병균실시형광PCR쾌속검험급소원분석방법,병재보장대형활동식품안전활동중추엄응용.방법채용“식품-찬음-초점의원”감측관리모식화실시형광 PCR 쾌속방법개전대남저시시수급대형활동중찬음중점식품중치병균화식원성병원주주동감측,응용PFGE분자분형방법개전소원분석,결합창신찬음관리모식,개전식품안전평고화예경.결과수부-남저시시수식품급찬음식품중균존재불동정도적부용혈성호균、사문씨균、지하씨균、장출혈성O157:H7、단핵세포증생리사특씨균、금황색포도구균、공장만곡균、판기장간균등치병균오염,기중이부용혈성호균검출솔최고체41.8%,기차시금황색포도구균위21.0%;복사표본중분리식원성치병균여식용식품유직접관계;식원성치병균위험평고여예경궤제건립급대형활동식품안전관리모식적응용유효감소료식원성질병발생.5년래,수부남저시재대형활동기간미발생식물중독사건,전시식물중독기수정축년하강추세;실시형광PCR화기인지문도보검측방법능구쾌속검측식품화복사표본중식원성치병균,통과산발성병례중적친연관계가소원도원인식품.결론“식품-찬음-초점의원”감측관리신모식적응용급검측신방법적사용가유효예방화공제식원성질병적발생,학보대형활동식품안전.
Objective To establish "Food-catering-sentinel hospitals" monitoring management mode and real-time fluorescence PCR as well as traceability method to ensure food safety in large-scale activities in Nanning City. Method A “food-catering-sentinel hospital” mode and real-time PCR method were adopted to implement active surveillance for food-borne pathogens in food samples from markets and important catering in large-scale activities in Nanning City. Food safety assessment and early warning were conducted by genetic fingerprinting of pathogens using PFGE in combination of the innovative catering management. Results There were varying degree contamination of V. parahaemolyticus, Salmonella, Shigella, O157:H7, M. Listeria, S. aureus,pathogens, C. jejuni, and E. sakazakii in commercially available food and catering food in Nanning City.. V. parahaemolyticus detection rate was the highest ( 41.8% ), followed by Staphylococcus aureus (21.0%); Foodborne pathogens isolated from diarrhea specimens were related to food directly; Risk assessment of foodborne pathogens, consumer warning mechanism and the food safety management model in large-scale activities could decrease the food borne diseases; Food poisoning incidents showed a declining trend and did not occur in events from 2005 to 2009 in Nanning City; Real-time fluorescence PCR could use to detect foodborne pathogens quickly and gene fingerprint detection methods could trace the dubious food that causes food-poisoning. Conclusion the "Food - catering - sentinel hospitals" monitoring management mode can effectively prevent and control foodborne diseases and ensure the food safety in large-scale activities in the provincial capital.
