CAJ | 학술논문

利用 ISSR 和 RAPD 技术对4个夏蜡梅种群25个单株的遗传多样性进行研究.从60条简单重复序列引物中筛选出了10条引物在25个个体中共检测到62个可重复的位点,其中多态位点为41个,总的多态位点百分率为65.60%;从60条寡居核苷酸引物中筛选出了10条引物共扩增出52个位点,其中多态性位点31个,多态性位点的百分率为57.50%. Shannon 指数估算的夏蜡梅群体总遗传多样性为0.3737,各群体平均遗传多样性为0.1645. Nei’s 指数估算的夏蜡梅群体总遗传多样性为0.2528,各群体平均遗传多样性为0畅1117;物种水平的有效等位基因数为1.4473,平均值为1.1972.4个自然群体的基因分化系数 Gst =0畅5297,即总的变异中有52.97%的变异存在于群体间,而群体内的遗传变异占总变异的47.03%,群体间遗传距离平均值0.2187.也表明了夏蜡梅4个群体间出现了遗传分化.夏蜡梅群体间的基因流较低,N m =0畅4450.聚类分析将4个种群聚为2支,且聚类结果表现出明显的地域性特征.作为狭域分布种,夏蜡梅各群体间存在遗传分化和多样性程度不高,对夏蜡梅种群多样性的就地和迁地保护势在必行.
이용 ISSR 화 RAPD 기술대4개하사매충군25개단주적유전다양성진행연구.종60조간단중복서렬인물중사선출료10조인물재25개개체중공검측도62개가중복적위점,기중다태위점위41개,총적다태위점백분솔위65.60%;종60조과거핵감산인물중사선출료10조인물공확증출52개위점,기중다태성위점31개,다태성위점적백분솔위57.50%. Shannon 지수고산적하사매군체총유전다양성위0.3737,각군체평균유전다양성위0.1645. Nei’s 지수고산적하사매군체총유전다양성위0.2528,각군체평균유전다양성위0창1117;물충수평적유효등위기인수위1.4473,평균치위1.1972.4개자연군체적기인분화계수 Gst =0창5297,즉총적변이중유52.97%적변이존재우군체간,이군체내적유전변이점총변이적47.03%,군체간유전거리평균치0.2187.야표명료하사매4개군체간출현료유전분화.하사매군체간적기인류교저,N m =0창4450.취류분석장4개충군취위2지,차취류결과표현출명현적지역성특정.작위협역분포충,하사매각군체간존재유전분화화다양성정도불고,대하사매충군다양성적취지화천지보호세재필행.
@@@@Genetic diversities of 4 populations 25 individuals of Calycanthus chinensis were studied by ISSR and RAPD marker .62 loci were identified with 10 simple sequence repeat primers screened from 60 primers ,out of which 41 loci were polymorphic and the percentage of polymorphic bands was 65 .60% ;52 loci were identified with 10 oligonucleotide primers screened from 60 primers ,out of which 31 loci were polymorphic and the percentage of polymorphic bands was 57 .50% .Shannon’s indices of diversity (I) were 0 .3737 among the species level with the average of 0.1645 among pop‐ulations .Nei’s gene diversity (h) was 0 .2528 among the species with the average of 0 .1117 among populations ;and the effective number of alleles (ne) was 1 .4473 among the species with the average of 1 .1972 among populations .Geneticvariance analysis showed the coefficient of gene differentiation (Gst) was 0 .5297 at species level ,and the gene flow (Nm ) was 0 .4450 .Cluster analysis indicated that 4 populations could be divided into 2 groups and consistent with their geo‐graphical distribution .As a highly restricted geographical distribution species ,the genetic diversity level of C .chinensis was not high .It is necessary to take measures to protect in situ or ex situ endangered species .