CAJ | 학술논문

硝酸还原酶是氮素代谢的关键酶和限速酶,研究硝酸还原酶的功能对提高甜菜的产量具有重要作用.运用 RACE 法克隆出甜菜的硝酸还原酶基因,并利用生物信息学对甜菜 NR 进行主要结构分析和功能预测,并使其在拟南芥中表达,观察根对向重性应答过程中的弯曲情况.结果表明,利用 RACE 法克隆得到甜菜NR cDNA 全长为2760 bp ;甜菜 NR 为易溶、亲水性强的蛋白;二级结构预测结果显示,甜菜 NR 为混合型蛋白;甜菜 NR 含有钼辅因子、细胞色素 b5、FAD 及 NADH 结合域,具有跨膜区域,但不含有信号肽;利用拟南芥突变体观察到野生型比突变体的弯曲较快,暗示甜菜的 NR 基因可能通过调节 NO 积累参与植物向重性应答.
초산환원매시담소대사적관건매화한속매,연구초산환원매적공능대제고첨채적산량구유중요작용.운용 RACE 법극륭출첨채적초산환원매기인,병이용생물신식학대첨채 NR 진행주요결구분석화공능예측,병사기재의남개중표체,관찰근대향중성응답과정중적만곡정황.결과표명,이용 RACE 법극륭득도첨채NR cDNA 전장위2760 bp ;첨채 NR 위역용、친수성강적단백;이급결구예측결과현시,첨채 NR 위혼합형단백;첨채 NR 함유목보인자、세포색소 b5、FAD 급 NADH 결합역,구유과막구역,단불함유신호태;이용의남개돌변체관찰도야생형비돌변체적만곡교쾌,암시첨채적 NR 기인가능통과조절 NO 적루삼여식물향중성응답.
@@@@Nitrate reductase is an important and limited enzyme in nitrogen metabolism .The functional research of ni‐trate reductase plays a key role in increasing sugar beet (Sb) yields .In this study ,SbNR gene was cloned by RACE and its 3D structure and physiological function were analysed by bioinformatics and expressed in Arabidopsis thaliana to show the gravity .The results were as follows :the full length of SbNR gene was 2760bp ;SbNR was soluble and hydrophobic ;secondary structure analyses showed that SbNR belonged to mixed protein ;SbNR was a new member of NR family ,containing molybdopterin‐binding ,cytochrome‐binding ,FAD‐binding and NAD‐binding domain .It had no signal peptide ,but one transmembrane domain in C terminal ,and the mutants showed later response to the gravity , and which confirmed that NO involved in plant’s gravity .