CAJ | 학술논문

目的研究血小板源性生长因子(PDGF)在小肠移植物中的表达及意义.方法利用显微外科技术构建同系和异系大鼠小肠移植模型,以同系移植组作为对照组,异系移植组作为实验组.分别在术后7 d、28 d、90 d进行移植物活检,常规病理学检测,应用Realtime-PCR技术检测移植物内PDGF mRNA转录水平,采用免疫组织化学方法对PDGF的表达进行定位.结果检测显示,所有移植物中PDGF mRNA转录水平总体呈上升趋势,到实验终点即POD90所采集的标本中以实验组的mRNA转录水平最高.免疫组化可见,PDGF在间质及小肠腺体内高表达.结论 PDGF因子在终末期小肠移植物中高表达,说明PDGF因子对于小肠移植慢性排斥反应有重要提示意义.
목적연구혈소판원성생장인자(PDGF)재소장이식물중적표체급의의.방법이용현미외과기술구건동계화이계대서소장이식모형,이동계이식조작위대조조,이계이식조작위실험조.분별재술후7 d、28 d、90 d진행이식물활검,상규병이학검측,응용Realtime-PCR기술검측이식물내PDGF mRNA전록수평,채용면역조직화학방법대PDGF적표체진행정위.결과검측현시,소유이식물중PDGF mRNA전록수평총체정상승추세,도실험종점즉POD90소채집적표본중이실험조적mRNA전록수평최고.면역조화가견,PDGF재간질급소장선체내고표체.결론 PDGF인자재종말기소장이식물중고표체,설명PDGF인자대우소장이식만성배척반응유중요제시의의.
Objective To analyse the expression of PDGF in small bowel transplantation in rats. Methods Isogeneic and allogeneic small bowel transplantation were performed in rats with microsurgical technology. Recipients were divided into two groups:isogeneic control group and allogeneic test group. Graft tissues were harvested on POD7, POD28 and POD90. mRNA transcription and immunohistochemical staining were also performed to detect the expression of PDGF. Results The expression of PDGF showed ascending tendency in whole stage, and the results of test group of POD90 were significantly higher than that of other stages. The immunohistochemical staining showed a higher expression in graft tissue in test group. Conclusion PDGF is highly expressed in the end stage of small bowel transplantation, and shows meaningful use for examination of chronic rejection.