CAJ | 학술논문

目的探讨LED-蓝光对大鼠肝大部切除肝再生后TNF-α、IL-6含量变化的影响.方法健康雄性Wistar大鼠24只,随机分为3组,假手术组、模型组及LED干预组.采用Higgins改良方法制备大鼠70%肝切除后肝再生的模型,在手术后的不同时间点,检测血清中TNF-α、IL-6的含量变化.结果各实验组动物在手术后1 d血清中TNF-α、IL-6的水平均有升高,模型组和LED干预组的TNF-α、IL-6水平均高于假手术组(P<0.05),且LED干预组的TNF-α、IL-6水平均显著高于模型组;在术后3 d假手术组血清TNF-α、IL-6水平即趋于正常,而模型组和LED干预组TNF-α、IL-6水平达高峰;在术后5 d和7 d,模型组和LED干预组血清中TNF-α、IL-6的水平开始下降,但LED干预组TNF-α、IL-6的水平仍高于模型组.结论LED-蓝光对肝切除后肝再生起明显的促进作用,其机制可能与增强TNF-α、IL-6的含量有关.
목적탐토LED-람광대대서간대부절제간재생후TNF-α、IL-6함량변화적영향.방법건강웅성Wistar대서24지,수궤분위3조,가수술조、모형조급LED간예조.채용Higgins개량방법제비대서70%간절제후간재생적모형,재수술후적불동시간점,검측혈청중TNF-α、IL-6적함량변화.결과각실험조동물재수술후1 d혈청중TNF-α、IL-6적수평균유승고,모형조화LED간예조적TNF-α、IL-6수평균고우가수술조(P<0.05),차LED간예조적TNF-α、IL-6수평균현저고우모형조;재술후3 d가수술조혈청TNF-α、IL-6수평즉추우정상,이모형조화LED간예조TNF-α、IL-6수평체고봉;재술후5 d화7 d,모형조화LED간예조혈청중TNF-α、IL-6적수평개시하강,단LED간예조TNF-α、IL-6적수평잉고우모형조.결론LED-람광대간절제후간재생기명현적촉진작용,기궤제가능여증강TNF-α、IL-6적함량유관.
Objective To investigate the effects of blue light-emitting diodes(LED) to the amount of TNF-αand IL-6 in the serum of liver regeneration rats after partial hepatectomy. Methods 24 Wistar rats were randomly divided into three groups:Sham group, Model group and LED group. The model was established by partial keratectomy and serum TNF-αand IL-6 levels at different time points were measured with ELISA. Results The levels of TNF-αand IL-6 in every group were increased at 1d after operation, and the levels of TNF-αand IL-6 in the groups of Model and LED were higher than those of in Sham group, but the levels of TNF-αand IL-6 in LED group were higher than those of in Model group (P<0.05). The levels of TNF-αand IL-6 increased gradually and reached a maximum level at 3d post operation in Model and LED group (P<0.05) and revered to normal in Sham group . Conclusion Blue LED can promote liver regeneration after partial hepatectomy, its effect is possibly related with increasing the amount of TNF-αand IL-6.