CAJ | 학술논문

目的观察复方甘草酸苷对免疫性肝损伤小鼠的保护作用.方法将50只小鼠随机分为正常对照组、模型组、复方甘草酸苷高、中、低剂量组[400、200、100 mg/(kg?d)],用药7 d,腹腔注射,正常对照组、模型组注射等量生理盐水,除正常对照组外,其余各组尾静脉注射20 mg/kg刀豆蛋白A(Con A).12 h后测定血清谷草转氨酶(AST)、谷丙转氨酶(ALT),计算肝脏、脾脏指数,测定血清TNF–α含量.结果与模型组比较,高、中、低剂量的复方甘草酸苷均能明显降低Con A介导的肝损伤小鼠肝、脾指数,降低血清中AST、ALT的水平,明显降低血清中TNF-α的含量.结论复方甘草酸苷对Con A所致免疫性肝损伤小鼠具有保护作用.
목적관찰복방감초산감대면역성간손상소서적보호작용.방법장50지소서수궤분위정상대조조、모형조、복방감초산감고、중、저제량조[400、200、100 mg/(kg?d)],용약7 d,복강주사,정상대조조、모형조주사등량생리염수,제정상대조조외,기여각조미정맥주사20 mg/kg도두단백A(Con A).12 h후측정혈청곡초전안매(AST)、곡병전안매(ALT),계산간장、비장지수,측정혈청TNF–α함량.결과여모형조비교,고、중、저제량적복방감초산감균능명현강저Con A개도적간손상소서간、비지수,강저혈청중AST、ALT적수평,명현강저혈청중TNF-α적함량.결론복방감초산감대Con A소치면역성간손상소서구유보호작용.
Objective To study the protective effect of compound glycyrrhizin (CG) on hepatic injury Induced by Con A. Methods 50 mice of BALB/C species were randomly divided into normal group, model group, high, medium, low-dose CG-treated group (400, 200, 100 mg?kg-1?d-1), CG-treated group were injected into peritoneal cavity, the normal and model groups were given normal saline. After 7 days, beside mice in normal group, other groups were injected with concanavalin A (Con A) 20 mg/kg via the tail vein to damage mice’s liver. After 12 hour, the index of liver and spleen were calculated .Using automatic biochemical analyzer, the alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum were examined, using ELISA for determination of the serum cytokines:tumor necrosis factor alpha (TNF-α). Results Compared with model group, high, medium, low-dose CG-treated group could significantly decrease the activity of AST and ALT and the index of liver and spleen in mice, and CG also could significantly reduce the inflammatory cytokines TNF-αlevel. Conclusion CG is effective in protecting immunological liver injury in mice induced by Con A.