CAJ | 학술논문

通过对日本蟳(Charybdis japonica)分别注射生理盐水(对照组)、溶藻弧菌(攻毒组)、免疫多糖(免疫组)、免疫多糖+溶藻弧菌(免疫攻毒组)后,再测定4组肝胰腺及肌肉中溶菌酶(LZM )和超氧化物岐化酶(SOD)的活性变化,探讨免疫多糖对日本蟳抗溶藻弧菌能力的影响.结果表明:日本蟳肝胰腺中 LZM 和SOD 的酶活均高于肌肉中的酶活,且免疫多糖对肝胰腺的免疫增强效应显著高于肌肉;免疫组肝胰腺和肌肉中 LZM 酶活,在注射后24 h 时显著升高至峰值(P<0.05),分别为(11.2±2.78)U /mg 和(3.75 ± 1.05)U /mg ,肝胰腺和肌肉中的 SOD 酶活也在24 h 达到最高值,分别是对照组的2.2倍和 1.4倍;免疫攻毒组在72 h时,肝胰腺和肌肉中的 LZM 酶活分别高出攻毒组62.8%和75.2%,两组织中 SOD 酶活同样显著高于攻毒组(P<0.05),是攻毒组的 3.5倍和4.2倍 . 由此可见,免疫多糖能提高日本蟳抗溶藻弧菌能力,且在72 h 达到最佳免疫保护效果.
통과대일본심(Charybdis japonica)분별주사생리염수(대조조)、용조호균(공독조)、면역다당(면역조)、면역다당+용조호균(면역공독조)후,재측정4조간이선급기육중용균매(LZM )화초양화물기화매(SOD)적활성변화,탐토면역다당대일본심항용조호균능력적영향.결과표명:일본심간이선중 LZM 화SOD 적매활균고우기육중적매활,차면역다당대간이선적면역증강효응현저고우기육;면역조간이선화기육중 LZM 매활,재주사후24 h 시현저승고지봉치(P<0.05),분별위(11.2±2.78)U /mg 화(3.75 ± 1.05)U /mg ,간이선화기육중적 SOD 매활야재24 h 체도최고치,분별시대조조적2.2배화 1.4배;면역공독조재72 h시,간이선화기육중적 LZM 매활분별고출공독조62.8%화75.2%,량조직중 SOD 매활동양현저고우공독조(P<0.05),시공독조적 3.5배화4.2배 . 유차가견,면역다당능제고일본심항용조호균능력,차재72 h 체도최가면역보호효과.
In order to explore influence of immunopolysaccharide on resistant to V ibrio alginolyticus ofCharybdis j aponica ,C .j aponicas were injected with saline(control group) ,V .alginolyticus (bacte‐rial infected group) ,immunopolysaccharide (immune group) ,immunopolysacchride and V . algino‐lyticus (immune plus bacterial infected group) ,and then the changes of the lysozyme (LZM ) and su‐peroxide dismutase (SOD) in the hepatopancreas and muscle were assaied .The results showed as fol‐lows the activities of LZM and SOD in hepatopancreas were higher than in the muscle ,and the im‐mune enhancement effect on the hepatopancreas was significantly higher than on the muscle . The LZM's activities of the immune group in the hepatopancreas and muscle were significantly elevated to the peak(P < 0 .05) after C .j aponica were injected at the 24h ,which were (11 .2 ± 2 .78)U /mg and (3 .75 ± 1 .05) U /mg .And SOD's activities of in the hepatopancreas and muscle also reached the peak at the 24h ,which were 2 .2 and 1 .4 times as high as that of control group .At the 72h ,LZM's activi‐ties in the hepatopancreas and muscle of the immune plus bacterial infected group were 62 .8% and 75 . 2% higher than those of the bacterial infected group .The SOD's activities were also significantly high‐er than those of bacterial infected group(P < 0 .05) ,which were 3 .5 and 4 .2 times as high as that of the bacterial infected group .Therefore ,immunopolysaccharide makes a effective influence on resistant to V ibrio alginolyticus of C .j aponica ,and it can get the optimal immune protection at 72h .