CAJ | 학술논문

对一种崭新的无载体酶固定化技术———聚集体(Cross‐linked Enzyme Aggregates ,CLEAs)技术进行了文献综述. CLEAs 技术是一种将蛋白质先沉淀后交联形成不溶性的、稳定的固定化酶.交联酶聚集体技术现已用于大量的合成反应.在沉淀和交联所需但具有潜在致变性化学品缺失的情况下,融合蛋白会将目的酶的相对选择性聚集体或其自组系统转为不溶性微粒,因而具有强劲的发展动力.在选定的生物转化中,用于多轮间歇式反应不溶蛋白颗粒的回收已经得到论证.然而,对于全连续生物催化过程应用来说,低耐机械应力和高压缩性依然是无载体酶颗粒所需要考虑的问题.
대일충참신적무재체매고정화기술———취집체(Cross‐linked Enzyme Aggregates ,CLEAs)기술진행료문헌종술. CLEAs 기술시일충장단백질선침정후교련형성불용성적、은정적고정화매.교련매취집체기술현이용우대량적합성반응.재침정화교련소수단구유잠재치변성화학품결실적정황하,융합단백회장목적매적상대선택성취집체혹기자조계통전위불용성미립,인이구유강경적발전동력.재선정적생물전화중,용우다륜간헐식반응불용단백과립적회수이경득도론증.연이,대우전련속생물최화과정응용래설,저내궤계응력화고압축성의연시무재체매과립소수요고필적문제.
Firstly ,a new carrier‐free immobilized enzyme technology‐aggregates ( Cross‐linked Enzyme Aggre‐gates ,CLEAs )technology has been reviewed .CLEAs technology is a kind of protein deposited before cross‐linking to form insoluble ,stability of the immobilized enzyme .Cross‐linked enzyme aggregates technology is now used in a large number of synthesis reaction .In the precipitation and cross‐linking required but has poten‐tial mutagenicity of chemicals is absent ,fusion protein will turn objective enzyme relatively selective aggregates or even its self‐organizing systems into insoluble particles ,which has a strong development momentum .In se‐lected biotransformation ,for many rounds of intermittent type reaction insoluble protein particles recycling has been demonstrated by .However ,for the continuous biocatalytic process applications ,low resistance to mechani‐cal stress and high compressibility are still the question that carrier‐free enzyme particles need to consider .