CAJ | 학술논문

为了扩大耐盐植物资源,促进沿海滩涂植物资源利用,以马齿苋(Portulaca oleracea L .)的嫩叶和茎段为试验材料,研究了不同浓度激素和 NaCl 对愈伤组织生长与分化的影响.结果表明:MS +0.10 mg?L‐1 NAA 时,马齿苋茎段生根率较高,生长健壮;MS +2.00 mg?L‐16‐BA 是不定芽诱导的最适培养基;MS +2.00 mg?L‐16‐BA +1.00 mg?L‐1 NAA 是分化的最适培养基;在最适分化培养基中添加100 mmol?L‐1 NaCl ,诱导产生的愈伤组织为5%,大部分愈伤组织生长受抑制,同时也说明马齿苋具有一定的耐盐性.
위료확대내염식물자원,촉진연해탄도식물자원이용,이마치현(Portulaca oleracea L .)적눈협화경단위시험재료,연구료불동농도격소화 NaCl 대유상조직생장여분화적영향.결과표명:MS +0.10 mg?L‐1 NAA 시,마치현경단생근솔교고,생장건장;MS +2.00 mg?L‐16‐BA 시불정아유도적최괄배양기;MS +2.00 mg?L‐16‐BA +1.00 mg?L‐1 NAA 시분화적최괄배양기;재최괄분화배양기중첨가100 mmol?L‐1 NaCl ,유도산생적유상조직위5%,대부분유상조직생장수억제,동시야설명마치현구유일정적내염성.
In order to expand salt‐tolerant plants ,promote the coastal beach plant resources utilization .With the stem and tender leaves of Portulaca oleracea L .as experimental material ,the effects of different concentration of 6‐BA and NAA and NaCl on growth and differentiation of callus were studied .The results showed that when MS + 0 .10 mg?L‐1 NAA ,the rooting of stem cutting was relatively high .MS + 2 .00 mg?L‐1 6‐BA was the opti‐mal medium for adventitious bud induction ;MS + 2 .00 mg?L‐1 6‐BA + 1 .00 mg?L‐1 NAA was the optimal medi‐um for differentiation .When stressed by 100 mmol?L‐1 NaCl on the optimal differentiation mediumn ,the callus induce frequency was only 5% and the formation of callus was inhibited ,it indicated that Portulaca oleracea L . had certain salt resistance .