CAJ | 학술논문

[目的]观察高甘油三脂血症对急性坏死性胰腺炎(ANP)大鼠肿瘤坏死因子α(TNF‐α)、白介素‐6(IL‐6)表达的影响,探讨高脂血症相关性 ANP 的特性.[方法]SD 大鼠54只,随机分成高脂血症+ ANP 模型组(A组),非高脂血症+ ANP 模型组(B 组),高脂血症+ 假手术模型组(C 组). A 、C 组通过高脂饲料喂养3周成高甘油三脂血症模型,A 、B 组大鼠建立 ANP 模型. A 、B 组分别成模后4 h 、8 h 、12 h 时点测定血甘油三脂(TG)、血淀粉酶(AMS)、TNF‐α和 IL‐6浓度,进行胰腺组织病理学评分;C 组大鼠假手术建模后同样方法检测以上指标.[结果]① A 组大鼠 ANP 成模前后 TG 浓度变化差异无统计学意义( P >0.05),B 组大鼠 ANP 成模后 TG 浓度较成模前增高( P <0.05). ② A 、B 组大鼠 ANP 成模后 TNF‐α和 IL‐6随时点逐渐增高,且 A 组大鼠 TNF‐α和IL‐6浓度明显高于 B 组( P <0.05). ③ A 组大鼠4 h 、8 h 、12 h 时点胰腺组织病理学评分明显高于 B 组( P <0.05),且与成模前 TG 浓度呈正相关.[结论]① 高甘油三脂血症时 TG 的浓度与 ANP 胰腺病理损害存在相关性;ANP 时可出现脂质代谢障碍.②高甘油三脂血症可上调 ANP 大鼠 TNF‐α和 IL‐6等炎症介质的释放.
[목적]관찰고감유삼지혈증대급성배사성이선염(ANP)대서종류배사인자α(TNF‐α)、백개소‐6(IL‐6)표체적영향,탐토고지혈증상관성 ANP 적특성.[방법]SD 대서54지,수궤분성고지혈증+ ANP 모형조(A조),비고지혈증+ ANP 모형조(B 조),고지혈증+ 가수술모형조(C 조). A 、C 조통과고지사료위양3주성고감유삼지혈증모형,A 、B 조대서건립 ANP 모형. A 、B 조분별성모후4 h 、8 h 、12 h 시점측정혈감유삼지(TG)、혈정분매(AMS)、TNF‐α화 IL‐6농도,진행이선조직병이학평분;C 조대서가수술건모후동양방법검측이상지표.[결과]① A 조대서 ANP 성모전후 TG 농도변화차이무통계학의의( P >0.05),B 조대서 ANP 성모후 TG 농도교성모전증고( P <0.05). ② A 、B 조대서 ANP 성모후 TNF‐α화 IL‐6수시점축점증고,차 A 조대서 TNF‐α화IL‐6농도명현고우 B 조( P <0.05). ③ A 조대서4 h 、8 h 、12 h 시점이선조직병이학평분명현고우 B 조( P <0.05),차여성모전 TG 농도정정상관.[결론]① 고감유삼지혈증시 TG 적농도여 ANP 이선병리손해존재상관성;ANP 시가출현지질대사장애.②고감유삼지혈증가상조 ANP 대서 TNF‐α화 IL‐6등염증개질적석방.
Objective] To observe the effect of hypertriglyceridemia on serum tumor necrosis factor ‐alpha(TNF‐α) and interleukin‐6(IL‐6) in rats with acute necrotizing pancreatitis(ANP) ,and to explore the characteristics of hypertrig‐lyceridemic‐related ANP .[Methods] A total of 54 SD rats were randomly divided into hypertriglyceridemia + ANP group (group A) ,ANP without hypertriglyceridemia group (group B) and hypertriglyceridemia + sham operation group (group C) .Group A and group C were made to be high triglyeride (TG) model by feeding with high‐fat diet for 3 weeks .Group A and group B were made to be ANP model .The levels of TG ,AMY ,TNF‐α and IL‐6 in group A and group B were measured at 4h ,8h and 12h after modeling .The pathology of pancreas was scored .Above indexes in group C after mod‐eling of sham operation were measured by using the same method .[Results] In group A ,there was no significant differ‐ence in TG level between before and after the modeling ( P > 0 .05) .Compared with before the modeling ,TG level in group B after modeling increased( P < 0 .05) .After modeling ,the levels of TNF‐α and IL‐6 in group A and group B in‐creased with the time gradually .The levels of TNF‐α and IL‐6 in group A were obviously higher than those in group B ( P < 0 .05) .The histological score of pancreas in group A at 4h ,8h and 12h was obviously higher than that in group B ( P < 0 .05) ,and positively related with TG level before modeling .[Conclusion] The level of TG in hypertriglyceridemia is correlated with histological damage of pancreas of ANP .Lipid metabolism occurs in ANP .Hypertriglyceridemia can up regulate the release of inflammatory mediator such as TNF‐α and IL‐6 in ANP rats .