CAJ | 학술논문

[目的]研究黄芩素与顺铂联合用药对人骨肉瘤 MG63细胞增殖和凋亡的影响.[方法]在骨肉瘤MG63细胞株中,分别采用 M TT 法检测黄芩素单独用药,与顺铂合用后细胞活性的变化情况;Hochest 染色、TUNEL 法检测药物单用及合用时细胞凋亡的变化情况.[结果]黄芩素30μM 对细胞活性没有明显影响,但在100μM 可时间依赖的降低细胞活性.黄芩素(100μM )与顺铂(3μM )合用后,能明显抑制细胞活性,具呈时效关系.黄芩素(100μM )可诱导细胞发生凋亡,黄芩素(30~100μM )与顺铂(3μM )两者合用具有协同诱导细胞凋亡的作用.[结论]黄芩素可抑制 MG63细胞活性,诱导凋亡;与顺铂合用具有协同效应.
[목적]연구황금소여순박연합용약대인골육류 MG63세포증식화조망적영향.[방법]재골육류MG63세포주중,분별채용 M TT 법검측황금소단독용약,여순박합용후세포활성적변화정황;Hochest 염색、TUNEL 법검측약물단용급합용시세포조망적변화정황.[결과]황금소30μM 대세포활성몰유명현영향,단재100μM 가시간의뢰적강저세포활성.황금소(100μM )여순박(3μM )합용후,능명현억제세포활성,구정시효관계.황금소(100μM )가유도세포발생조망,황금소(30~100μM )여순박(3μM )량자합용구유협동유도세포조망적작용.[결론]황금소가억제 MG63세포활성,유도조망;여순박합용구유협동효응.
Objective To explore the effect of baicalein combined with cisplatin on cell proliferation and apopto ‐sis of human osteosarcoma cells MG63 .[Methods] Human osteosarcoma MG63 cells were treated with baicalein or baicalein combined with cisplatin .The change of cell activity was measured by MTT assay .Cell apoptosis was detec‐ted by Hochest staining and TUNEL method .[Results] Baicalein 30 M had no effect on cell activity ,while baicalein 100 M decreased cell activity in a time‐dependent manner .Baicalein 100 M combined with cisplatin 30 M could obvi‐ously decrease cell activity and had synergistic effect .Baicalein 100 M could induce cell apoptosis .Baicalein 30 ~ 100 M combined with cisplatin 3 M had synergistic effect on inducing cell apoptosis .[Conclusion] Baicalein can inhibit MG63 cell activity and induce cell apoptosis .Baicalein combined with cisplatin has synergistic effect .