CAJ | 학술논문

[目的]研究炎症性肠病患者血浆早老蛋白‐1(Presenilin‐1)的表达和对溃疡性结肠炎及克罗恩病的鉴别诊断价值.[方法]应用酶联免疫吸附试验(ELISA)分析克罗恩病患者、溃疡性结肠炎患者和正常对照者血浆中 Presenilin 1的表达水平.通过受试者工作特征(receiver‐operating characteristic ,ROC)曲线观察血浆 Presenilin‐1水平对溃疡性结肠炎患者和克罗恩病患者的鉴别诊断价值.[结果]溃疡性结肠炎患者血浆Presenilin‐1水平显著高于正常对照者( P <0.01)和克罗恩病患者( P =0.0053),同时血浆对 Presenilin‐1水平对克罗恩病患者和溃疡性结肠炎患者具有显著的鉴别价值(AUC =0.7657,P <0.01),但是溃疡性结肠炎患者血浆 Presenilin‐1与内镜疾病活动性无显著相关性(Spearman r =0.1224,P =0.4022).[结论]溃疡性结肠炎患者血浆 Presenilin‐1表达水平增高,可能对鉴别诊断克罗恩病有一定价值.
[목적]연구염증성장병환자혈장조로단백‐1(Presenilin‐1)적표체화대궤양성결장염급극라은병적감별진단개치.[방법]응용매련면역흡부시험(ELISA)분석극라은병환자、궤양성결장염환자화정상대조자혈장중 Presenilin 1적표체수평.통과수시자공작특정(receiver‐operating characteristic ,ROC)곡선관찰혈장 Presenilin‐1수평대궤양성결장염환자화극라은병환자적감별진단개치.[결과]궤양성결장염환자혈장Presenilin‐1수평현저고우정상대조자( P <0.01)화극라은병환자( P =0.0053),동시혈장대 Presenilin‐1수평대극라은병환자화궤양성결장염환자구유현저적감별개치(AUC =0.7657,P <0.01),단시궤양성결장염환자혈장 Presenilin‐1여내경질병활동성무현저상관성(Spearman r =0.1224,P =0.4022).[결론]궤양성결장염환자혈장 Presenilin‐1표체수평증고,가능대감별진단극라은병유일정개치.
the plasma expression of Presenilin 1 in patients with inflammatory bowel disease and its value in the differenti‐al diagnosis of ulcerative colitis and Crohn's disease .[Methods] Enzyme‐linked immunosorbent assay(ELISA) was applied to analyze plasma level of Presenilin‐1 in patients with Crohn's disease ,ulcerative colitis and healthy controls .Receiver operating characteristic(ROC) curve was used to observe differential diagnostic val‐ue of plasma level of Presenilin‐1 in patients with ulcerative colitis and Crohn's disease .GraphPad Prism 5 .0 was used for the data processing .[Results] Plasma level of Presenilin‐1 in patients with ulcerative colitis was significantly higher than that in normal controls ( P < 0 .01) and patients with Crohn's disease( P = 0 .0053) . Plasma level of Presenilin‐1 had significant diagnostic value in patients with ulcerative colitis and Crohn's dis‐ease (AUC = 0 .7657 ,P < 0 .01) .However ,there was no significant correlation between plasma level of Pre‐senilin‐1 and endoscopic disease activity in patients with ulcerative colitis (Spearman r = 0 .1224 ,P = 0 .4022) .[Conclusion] Plasma level of Presenilin‐1 in patients with ulcerative colitis increased ,which may contribute to the differential diagnosis of ulcerative colitis and Crohn's disease .